Production of γ-linolenic acid in oleaginous yeast

Details Production of γ-linolenic acid in oleaginous yeast Production of γ-linolenic acid in oleaginous yeast

2005-08-08

2008-12-16

Woitach, Joseph (Department: 1633)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S254200, C435S252300, C435S069200, C536S023200

Reexamination Certificate

active

07465564

ABSTRACT:
The present invention relates to methods for the production of γ-linolenic acid (GLA) in oleaginous yeast. Thus, Δ12 and Δ6 desaturases able to catalyze the conversion of oleic acid to GLA have been introduced into the genome ofYarrowia, using zeta-directed integration. Transformed strains produced over 25% GLA in the total lipids, as opposed to wildtypeYarrowiathat is unable to synthesize any GLA. Metabolic engineering and fermentation methods are provided to further enhance GLA productivity in oleaginous yeast.

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