Compositions and methods for cDNA synthesis

Details Compositions and methods for cDNA synthesis Compositions and methods for cDNA synthesis

2005-09-29

2008-12-30

Chunduru, Suryaprabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091210, C536S024330

Reexamination Certificate

active

07470515

ABSTRACT:
Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

REFERENCES:
patent: 5561058 (1996-10-01), Gelfand et al.
patent: 5614387 (1997-03-01), Shen et al.
patent: 5693517 (1997-12-01), Gelfand et al.
patent: 5919900 (1999-07-01), Moyle et al.
patent: 6087489 (2000-07-01), Dean
patent: 6214542 (2001-04-01), Striker et al.
patent: 6300069 (2001-10-01), Missel et al.
patent: 6300073 (2001-10-01), Zhao et al.
patent: 6406891 (2002-06-01), Legerski
patent: 6416989 (2002-07-01), Abad et al.
patent: 6495350 (2002-12-01), Lee et al.
patent: 6521411 (2003-02-01), Hecker et al.
patent: 6767724 (2004-07-01), Lee et al.
patent: 01/00820 (2001-01-01), None
patent: WO 01/00820 (2001-01-01), None
Peng et al. Multiple PCR analyses on trace amounts of DNA extracted from fresh and paraffin wax embedded tissues after random hexamer primer PCR amplification. J Clin Pathol., vol. 47, p. 605-608, 1994.
Boehringer Mannheim 1997 Biochemicals Catalog, pp. 146-147, 150-153, and an instruction Manual from Tital RT-PCR system, Cat. No. 1855476.
“Protocols and Applications Guide, Third Edition”, Promega Protocols and Applications Guide, 1996, pp. 181-189.
“Stratagene Catalog: RT-PCR Systems and Kits”, Stratagene Calalogue, 1999, pp. 154-155, XP002264374.

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