Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1984-10-17
1987-08-11
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 23, 435 28, 435 29, 435810, 435 25, 436 23, C12Q 136, C12Q 138, C12Q 102
Patent
active
046861823
ABSTRACT:
A method of detecting a beta-lactam antibiotic in a liquid sample comprising the steps of: (a) forming a reaction medium by adding the sample to a predetermined amount of a dry reagent formulation comprising a D,D-carboxypeptidase, a substrate for said D,D-carboxypeptidase containing a carboxy-terminal D-alanine, and a reagent system which produces a color change in the presence of D-alanine; (b) incubating said reaction medium for a predetermined period; (c) adding a quenching material to terminate the reaction; (d) observing the color of the reaction medium. A kit for carrying out this method is also disclosed.
REFERENCES:
patent: 4239745 (1980-12-01), Charm
patent: 4239852 (1980-12-01), Charm
patent: 4331761 (1982-05-01), Dawson et al.
patent: 4427632 (1984-01-01), Okaniwa et al.
patent: 4442204 (1984-04-01), Greenquist et al.
patent: 4546076 (1985-10-01), Degelaen et al.
patent: 4555484 (1985-11-01), LaRossa et al.
Frere, J. et al, Biochem J., vol. 143, 1974, pp. 233-240.
Chemical Abstracts, vol. 90, No. 25, 1979, p. 227, #199525m, Frere, J. M. et al, "The Exocellular . . . with .beta.-Lactam Antibiotics".
Chemical Abstracts, vol. 85, No. 23, 1976, p. 180, #173261u, Marquet, A. et al, "Membrane-Bound . . . .beta.-Lactam Antibiotics".
Chemical Abstracts, vol. 87, No. 7, 1977, p. 73, #48470b, "Interactions between . . . Streptococcus Faecalis, ATCC 9790".
Chemical Abstracts, vol. 83, No. 17, 1975, p. 199, Abstract No. 143667x, Kawashima, K. et al.
Frere, J. et al, "Enzymatic Method for Rapid and Sensitive Determination of .beta.-Lactam Antibiotics", Antimicrobial Agents and Chemotherapy, vol. 18, No. 4, Oct. 1980, pp. 506-510.
Fuad, N. et al, Biochemical J., vol. 155, 1976, pp. 623-629.
Allen Mary M.
Jay Jeremy M.
Marantz Sidney
Minnesota Mining and Manufacturing Company
Sell Donald M.
LandOfFree
Method and kit for detecting antibiotics in a liquid sample does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and kit for detecting antibiotics in a liquid sample, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for detecting antibiotics in a liquid sample will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-402773