Production of the lipidated form of the peptidoglycan...

Details Production of the lipidated form of the peptidoglycan... Production of the lipidated form of the peptidoglycan...

2000-06-20

2008-11-18

Duffy, Patricia A (Department: 1645)

Chemistry: molecular biology and microbiology

Vector, per se

C435S252300

Reexamination Certificate

active

07452715

ABSTRACT:
The expression of the lipidated form of the peptidoglycan-associated protein (PAL) of gram-negative bacteria is achieved through the use of a plasmid containing a tightly regulated promoter. A bacterial host cell is transformed, transduced or transfected with such a plasmid. The host cell is then cultured under conditions such that the lipidated recombinant PAL is expressed. The lipidated recombinant PAL is included in an antigenic composition administered to a mammalian host to immunize against a gram-negative bacterium.

REFERENCES:
patent: WO/90 02557 (1990-03-01), None
Baneyx (Current Opinion in Biotechnology, 10:411-421, 1999).
Makrides et al (Microbiological Reveiws, 60(3):512-538, Sep. 1996).
Green, B.A., et al.,Infect Immun., 59, (3191-3198), (1991).
Green, B.A., et al.,Infect. Immun., 58, (3272-3278), (1990).
Zlotnick, G.W., et al.,J. Biol. Chem., 263, (9790-9794 ), (1988).
Lim, A., Jr., et al.,Microbiology, 143, (1709-1716), (1997).
Guzman, L.M., et al.,J. Bacteriol., 177, (4121-4130), (1995).
Mertens, N., et al.,Gene, 16, (9-15), (1995).
Mertens, N., et al.,Biotechnology(N.Y.), 13 (175-179), (1995).
Chandrashekharan, S., et al.,Biol. Chem., 379, (579-582), (1998).
Reddy, P. et al.,Nucleic Acids Res., 25, (10473-10488), (1989).
Sagawa, H., et al.,Gene, 168, (37-41), (1996).
Waleh, N. S.Gene, 117, (7-14), (1992).
Skerra, A.,Gene, 151, (131-5), (1994).
Haldimann, A., et al.,J. Bacteriol., 180, (1277-1286), (1998).
Lutz, R., et al.,Nucleic Acids Res., 25, (1203-1210), (1997).
Wulfing, C.,Gene, 136, (199-203), (1993).
Nelson, M. B., et al., “Cloning and sequencing ofHaemophilus influenzaeouter membrane protein P6”.Infection and Immunity, vol. 56, No. 1, Jan. 1988, pp. 128-134.
Yang, Y.P. et al: Effect of lipid modification on the physicochemical, structural, antigenic and immunoprotective properties ofHaemophilus influenzaeouter membrane protein P6:Vaccine GB, Butterworth Scientific Guildford, vol. 15, No. 9, Jun. 1, 1997, pp. 976-987.

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