Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1987-04-20
1989-06-06
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 18, 436 64, 436 71, 436 93, 436 94, 436164, 436175, 436177, C12Q 100, C12Q 134, G01N 2178, G01N 3102
Patent
active
048371440
ABSTRACT:
A method of measuring lipid-bound sialic acid (LSA) which comprises, mixing a LSA separating agent comprising a polar solvent with a sample containing LSA and other sialic acid(s), separating the precipitate formed to obtain a supernatant, and measuring the sialic acid concentration of said supernatant by using an enzyme capable of acting on sialic acid in a lipid-bound state. When a sample is treated with the LSA separating agent comprising a polar solvent, various components in the sample such as protein-bound sialic acid and proteinous substances precipitate while the desired lipid-bound sialic acid remains in the supernatent. Accordingly, by measuring sialic acid concentration of the supernatent, LSA concentration of the sample is easily determined. The method of the invention is simple and superior in reproducibility. The method of the invention is suitable for treating a large number of samples, and it can easily by automated. This method is suitable as a screening test for cancer.
REFERENCES:
patent: 4115062 (1978-09-01), Morre et al.
patent: 4342567 (1982-08-01), Katopodis et al.
patent: 4457865 (1984-07-01), Miller
patent: 4493898 (1985-01-01), Sallay
patent: 4520111 (1985-05-01), Miller
patent: 4701418 (1987-10-01), Katopodis
patent: 4748128 (1988-05-01), Katopodis
Sugahara et al., Clin. Chim. Acta, vol. 108, pp. 493-498, 1980.
"Improved Method to Determine Lipid Bound Sialic Acid In Plasma or Serum", Katopodis et al., Research Comm. in Chem. Path. & Pharmacology, vol. 30, No. 1, Oct. 1980.
J. Jpn. Soc. Cancer Ther. 18(3): 692-703, Apr., 1983 "Total Sialic Acid and Lipid Bound Sialic Acid in Sera of Digestive Cancer Patients", Oki.
Laboratory Techniques in Biochemistry & Molecular Biology, "Techniques of Lipidology", Kates, 1972, Chapter 3, pp. 347-353.
Fujirebio Kabushiki Kaisha, also trading as Fujirebio Inc.
Hill Jr. Robert J.
LandOfFree
Method of measuring lipid-bound sialic acid does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of measuring lipid-bound sialic acid, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of measuring lipid-bound sialic acid will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-40235