Signal sequences for preparing leu-hirudin by secretion by...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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Reexamination Certificate

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07455987

ABSTRACT:
The present invention relates to a efficient expression of a protein, particularly, hirudin; a protein precursor comprising a signal sequence and the sequence of aaX-hirudin, wherein aaXis a selected amino acid, and aaXis preferably leucine; to its preparation and use; and to processes for finding signal sequences for secretory expression of any desired protein inE coli; and to processes for the secretory expression of any desired protein inE coli.

REFERENCES:
patent: 5180668 (1993-01-01), Crause et al.
patent: 5286714 (1994-02-01), Crause et al.
patent: 5316947 (1994-05-01), Crause et al.
patent: 5389529 (1995-02-01), Panayotatos et al.
patent: 5616476 (1997-04-01), Crause et al.
patent: 5652139 (1997-07-01), Wong et al.
patent: 5919895 (1999-07-01), Schmid et al.
patent: 0 324 712 (1989-07-01), None
patent: 0 448 093 (1991-09-01), None
patent: 0 511 393 (1992-11-01), None
patent: 0 549 915 (1993-07-01), None
Achstetter et al. A new signal peptide useful for secretion of heterologous proteins from yeast and its application for synthesis hirudin. (1992) Gene 110: 25-31.
International Search Report, dated Feb. 28, 2001.
R. De Mot et al., “Homology of the root adhesion ofPseudomonas fluorescensOE 28.3 with porin F ofP. aeruginosaandP. syringae,” Mol. Gen. Genet, 231:489-493 (1992).
G. Braum and S.T. Cole, “DNA sequence analysis of theSerratia marcescens ompAgene: Implications for the organisation of an enterobacterial outer membrane protein,”Mol. Gen. Genet, 195:321-328 (1984).
Sara L. Pealing et al., “Sequence of the Gene Encoding FlavocytochromecfromShewanella putrefaciens: A Tetraheme Flavoenzyme That Is a Soluble Fumarate Reductase Related to the Membrane-Bound Enzymes from Other Bacteria,”Biochemistry, 31:12132-12140 (1992).
esp@cenet summary for EP 0 549 915 published Jul. 7, 1993.

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