Padlock probe detection

Details Padlock probe detection Padlock probe detection

1996-06-14

1999-06-15

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07K 2102, C07K 2104, C12N 1500

Patent

active

059121246

ABSTRACT:
The present invention relates to the detection of a polynucleotide using a padlock probe, whereby a hybridization process is combined with ligation to irreversibly capture polynucleotides of interest. The invention relates both to the padlock probe itself, as well as to methods of use, which allow for varying levels of sensitivity due to amplification of signal processes built into the system. Additionally, preferred formats are disclosed for use of the padlock probes, including the use of microfluidics.

REFERENCES:
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5359115 (1994-10-01), Campbell et al.
patent: 5420328 (1995-05-01), Campbell
patent: 5424186 (1995-06-01), Fodor et al.
Bardin et al., Histochemistry 100: 387-392 (1993).
Livak et al., PCR Methods and Applications 4: 357-362 (1995).
Landegren et al., Science 241: 1077-1080 (1988).
Matthews et al. Anal. Biochemistry 169: 1-25 (1988).
Agrawal et al., Nucleic Acids Research 18(18):5419-5423 (1990).
The DYNAL Technical Handbook, 2nd Edition, pp. 136-137 (1995).
Lee et al., Virus Genes, 9:177-181, 1994.
Nilsson et al., Science, 265:2085-2088 (1994).
Walker et al., "The Language of Biotechnology: A dictionary of terms" published by the American Chemical Society (1988).

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