Protein profiles with atmospheric pressure ionization

Details Protein profiles with atmospheric pressure ionization Protein profiles with atmospheric pressure ionization

2005-10-21

2008-10-28

Berman, Jack I. (Department: 2881)

Radiant energy

Ionic separation or analysis

Methods

C250S290000, C250S288000, C250S292000, C250S293000, C250S284000, C250S287000, C250S424000, C702S023000, C702S027000, C702S104000, C435S006120, C435S007100, C435S007230, C435S174000

Reexamination Certificate

active

07442921

ABSTRACT:
The invention relates to the acquisition of mass spectra of complex protein mixtures, often called protein profiles, for example to search for biomarkers which indicate stress situations, or to identify microbes. Up to now protein profiles have been acquired using ionization by matrix-assisted laser desorption with high detection sensitivity in linear time-of-flight mass spectrometers, but these display very poor mass resolution and a very poor reproducibility of the mass values. The invention provides methods which produce surprisingly similar mass spectra, but with far higher mass resolution and mass accuracy. Ionization takes place outside the vacuum at ambient pressure, preferably by means of laser desorption and CI post-ionization. Analysis of the ions takes place in a high-resolution mass spectrometer, for example a reflector time-of-flight mass spectrometer with orthogonal ion injection.

REFERENCES:
patent: 5373156 (1994-12-01), Franzen
patent: 5663561 (1997-09-01), Franzen et al.
patent: 5965884 (1999-10-01), Laiko et al.
patent: 6683300 (2004-01-01), Doroshenko et al.
patent: 6707037 (2004-03-01), Whitehouse
patent: 7022981 (2006-04-01), Kato
patent: 2003/0052268 (2003-03-01), Doroshenko et al.
patent: 2004/0089802 (2004-05-01), Kato
patent: 42 02 123 (1992-01-01), None
patent: 196 08 963 (1996-03-01), None
patent: 196 17 011 (1996-04-01), None
patent: 196 08 963 (1996-10-01), None
patent: 199 34 173 (1999-07-01), None
patent: 199 34 173 (2001-01-01), None
patent: 10 2004 002 729 (2004-01-01), None
patent: 0 964 417 (1999-12-01), None
patent: 0 964 427 (1999-12-01), None
patent: 0 966 022 (1999-12-01), None
patent: 2 299 445 (1996-03-01), None
patent: 2 310 950 (1997-09-01), None
patent: WO 98/00224 (1998-01-01), None
patent: WO 99/38185 (1999-07-01), None
patent: WO 03/102508 (2003-12-01), None
patent: WO 2004/030024 (2004-04-01), None

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