Method of forming vertebrate pancreas in vitro

Details Method of forming vertebrate pancreas in vitro Method of forming vertebrate pancreas in vitro

2001-03-13

2008-08-19

Barnhart, Lora E (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S325000, C435S375000, C424S093100, C623S023720

Reexamination Certificate

active

07413901

ABSTRACT:
The present invention provides a pancreas induced in vitro that is useful for developmental engineering and organ engineering, and a method wherein a pancreas induced in vitro which contributes to the development of diagnosis and treatment of pancreatic disorders for higher animals, can artificially and efficiently be induced from a gastrula apart from the presumptive region of pancreas. An explant which has a secretory gland-like structure wherein several cells are gathered and which expresses insulin is formed in vitro by treating the blastopore upper lip of an early gastrula of a vertebrate such asXenopuswith retinoic acid in vitro, and then culturing. The treatment with retinoic acid can be carried out, for example, by treating with retinoic acid at a concentration of 10−5M or above for three hours.

REFERENCES:
patent: 2000 217571 (2000-08-01), None
patent: WO00 44881 (2000-08-01), None
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