Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2003-11-04
2008-08-26
Epps-Ford, Janet L. (Department: 1633)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C536S023100, C536S024100, C435S325000, C800S004000, C800S008000, C800S021000
Reexamination Certificate
active
07416886
ABSTRACT:
The present invention relates to a porcine uroplakin II gene promoter, an expression vector containing the promoter, and a method for producing useful proteins using the vector. The promoter of the present invention promotes the bladder-specific expression of a target protein at high efficiency. An animal, which was transformed using the inventive promoter so as to express the target protein, secretes the target protein in its urine at high concentration, and the protein thus produced shows a superior physiological activity to that of the same kind of the existing protein. As a result, the inventive promoter, the expression vector and transgenic animal using the promoter, can be advantageously used in the production field of useful proteins that are medicinally valuable.
REFERENCES:
patent: 6001646 (1999-12-01), Sun
patent: 6339183 (2002-01-01), Sun
patent: 2002/0092033 (2002-07-01), Sun
Kwon et al (Cloning, Sequencing and Expression Analysis of the Porcine uroplakin II gene, Biochemical and Biophysical Research Communications, 2002. 293:862-869).
Zbikowska et al (The use of the uromodulin promoter to target production of recombinant proteins into urine of transgenic animals, Transgenic Research, 2002. 11:425-435).
Kwon et al (Cloning and Molecular Dissection of the 8.8 kb Pig Uroplakin II Promoter Using Transgenic Mice and RT4 cells, Journal of Cellular Biochemistry, 2006. 99:462-477).
Robl et al (Transgenic animal production and animal biotechnology, Theriogenology, 2007. 67:127-133).
D. N. Kwon et al., “Cloning, sequencing, and expression analysis of the porcine uroplakin II gene,” Biochemistry and Biophysical Research Communication, vol. 293, No. 2, pp. 862-869 (2002).
NCBI GeneBank Association No. AY044189, 4 pages including one sequence listing, (2001).
Takahito Inoue et al., “Position-independent human β-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken β-globin insulator,” J. Hum Genet, vol. 44, No. 3, pp. 152-162 (1999).
Romain Zufferey et al., “Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of Transgenes Delivered by Retroviral Vectors,” Journal of Virology, vol. 73, No. 4, pp. 2886-2892 (1999).
Lin et al., “Cloning and expression of the human erythropoietin gene,” Proc. Natl. Acad. Sci USA, 82:7580-7584 (1985).
Cho-A Pharm Co., Ltd.
Epps-Ford Janet L.
Greenblum & Bernstein P.L.C.
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