Purification of immunoglobulins using affinity...

Details Purification of immunoglobulins using affinity... Purification of immunoglobulins using affinity...

2005-01-13

2008-08-05

Saunders, David A (Department: 1644)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

6 to 7 amino acid residues in defined sequence

C530S413000

Reexamination Certificate

active

07408030

ABSTRACT:
An immunoglobulin binding peptide having the general formula, from amino terminus to carboxy terminus, of Z-R1—R2—R3—R4—R5—R6—X, is described, wherein: R1is H or Y; R2is a hydrophobic, preferentially aromatic, amino acid (for example W, F, Y, V); R3is a positively charged or aromatic amino acid (for example R, H, F, W); R4is a hydrophobic or positively charged amino acid (for example G, Y, R, K, L); R5is a positively charged or aromatic amino acid (for example W, F, R, H, Y); R6a random amino acid but preferably hydrophobic or negatively charged (for example V, W, L, D, H); X is present or absent and when present is a linking group; and Z is present or absent and when present is a capping group bonded to the N terminus of R1; and wherein the amino acids of said peptide are in D form, L form, or a combination thereof. Methods of using such peptides for the purification of Immunoglobulins are also described.

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Tosoh Bioscience product catalog, “Affinity Chromatography” (no date), cover and pp. 38-42. Tosoh Bioscience, Montgomeryville, PA.
Kato Y, et al., “High-performance metal chelate affinity chromatography of proteins,” Journal of Chromatography (1986) vol. 354, pp. 511-517.
Gurgel P V, et al., “Identification of peptide ligands generated by combinatorial chemistry that bind alpha-lactalburnin,” Separation Science and Technology (2001), vol. 36(11), pp. 2411-2431, Marcel Dekker, Inc., NY, NY.

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