Method for the identification of tissue-specific regulatory...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S320100, C435S252300, C435S252800

Reexamination Certificate

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07425429

ABSTRACT:
The invention relates to a method for the identification of regulatory sequences in genomes of micro-organisms, isolated ex vivo from infected tissues. The invention further relates to promoters in salmonellae identified by said method.

REFERENCES:
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patent: 6010705 (2000-01-01), Thune et al.
Sampson et al., Expert. Rev. vaccines, vol. 2, 2003, pp. 437-445.
Sheela et al., Clinical and Diagnostic Laboratory Immunology, vol. 10, 2003, pp. 670-679.
McClelland et al., “Complete genome sequence of Salmonella enterica serovar Typimurium LT2”, Nature, vol. 413, No. 6858, Oct. 25, 2001, pp. 852-856.
Dunstan et al., “Use of in vivo-regulated promoters to deliver antigens from attenuated Salmonella enterica var. Typhimurium”, Infection and Immunity, American Society for Microbiology, vol. 67, No. 10, Oct. 1999, pp. 5133-5141.
Bumann et al., “Regulated antigen expression in live recombinant Salmonella enterica serovar Typhimurium strongly affects colonization capabilities and specific CD4(+)−T-cell response.”, Infection and Immunity. vol. 69, No. 12, Dec. 2001, pp. 7493-7500.

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