Solution for the storage and perfusion of organs awaiting...

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

Reexamination Certificate

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Reexamination Certificate

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07422844

ABSTRACT:
A storage solution to maintain and perfuse organs awaiting transplantation comprising (a) an isotonic balanced solution comprising a physiologically acceptable amount of potassium, mono acidic phosphate, biacidic phosphate, chloride, sodium and bicarbonate ions; (b) 50-250 mM glucose; (c) 0.2-20 mM of an alkanoyl L-carnitine or a physiologically acceptable salt thereof; (d) 1-100 mM of L-carnitine or a physiologically acceptable salt thereof; (e) water is described. The storage solution can also include other components such as anti-oxidants and/or chelating agents.

REFERENCES:
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patent: RE36331 (1999-10-01), Arduini
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patent: 96/40167 (1996-12-01), None
patent: 00/30637 (2000-06-01), None
patent: 00/30637 (2000-06-01), None
Loster et al., “Effects of L-camitine and its acetyl and propionyl esters on ATP and PCr levels of isolated rat hearts perfused without fatty acids and investigated by means of 31P-NMR spectorscopy”, Molecular and Cellular Biochemistry 200 : 93-102 (1999).
Boehmova et al., “Renal ischemia-reperfusion injury: an inescapable event affecting kidney transplantation outcome”, Folia Microbiologica 46 (4) : 267-276 (2001).
Kasperk et al., “Morphometric study of centrilobular vessels in the rat liver after continuous perfusion with Euro-Collins and University of Wisconsin solution”, Research in Experimental Medicine 194 (6) : 383-90 (1994).
Rossaro et al., “Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hyptothermic perfusion”, Journal of Laboratory and Clinical Medicine 120 (4) : 559-68 (1992).
Database Biosis ′Online; Biosciences Information Service, Philadelphia, PA, US: 1990, Klepetko et al; Pulmonary Surfactant in Bronchoalveolar Lavage After Canine Lung Transplantation Effect of L Carnitine Application; Database Accession No. PREV199090067674; XP002215925.
Database Medline ′Online; Apr. 1994; Nakagawa et al; “The Effect of L-Carnitine on Myocardial Protection in Coid Cardioplegia Followed By Reperfusion”; Database Accession No. NLM8016834; XP002215926.
O. Ergun et al; “Carnitine as Preventive Agent in Experimental Renal Ischemia-Reperfusion Injury”; Urological Research, vol. 29, No. 3, Jun. 11, 2001, pp. 186-189, XP002215923.
U. Puetz et al; “Effects of L-Carnitine-Hydrochlorine in the Cold Ischemic Preservation of Fatty Liver Grafts”; Transplantion Proceedings, vol. 33, 2001, pp. 2523-2524, XP002215924.
Database Biosis Online; Biosciences Information Service, Philadelphia, PA, US; Mar. 2002, Mister Marilena et al; “Propionyl-L-Carnitine Prevents Renal Function Deterioration Due to Ischemia/Reperfusion”; Database Accession No. PREV200200206973, XP002215927.
Database Medline ′Online, 1992, Reckendorfer et al; “Hepatic Energy Metabolism During Hypothermic Storage and Reperfusion Using Different Protecting Solutions”; Database Accession No. NLM1292938, XP002215928.
Database CA ′Online; Chemical Abstracts Service, Columbus, Ohio, US; S. Skrede et al; “Coenzyme a in Dog Kidneys During Hypothermic Perfusion”; Retrieved From STN-International Database Accession No. 99:67125 CA, XP002215929, 1983.

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