Methods and kits for indirect labeling of nucleic acids

Details Methods and kits for indirect labeling of nucleic acids Methods and kits for indirect labeling of nucleic acids

2008-05-13

2008-05-13

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091500

Reexamination Certificate

active

10431335

ABSTRACT:
Methods and kits for labeling nucleic acids are provided. In the subject methods, an oligonucleotide tagged nucleic acid comprising an oligonucleotide tag is first generated. The oligonucleotide tagged nucleic acid is then contacted under hybridization conditions with a labeled oligonucleotide complementary to the oligonucleotide tag, yielding a labeled nucleic acid. The kits of the subject invention at least include a primer for use in enzymatically generating an oligonucleotide tagged target nucleic acid, where the primer generally at least includes an oligo dT region and the oligonucleotide tag, and a labeled oligonucleotide complementary to the oligonucleotide tag. The subject methods and kits find use in a variety of applications, and are particularly suited for use in gene expression analysis applications.

REFERENCES:
patent: 5175270 (1992-12-01), Nilsen et al.
patent: 5484904 (1996-01-01), Nilsen et al.
patent: 5487973 (1996-01-01), Nilsen et al.
patent: 5574141 (1996-11-01), Seliger et al.
patent: 5604097 (1997-02-01), Brenner
patent: 5635400 (1997-06-01), Brenner
patent: 5695934 (1997-12-01), Brenner
patent: 5716785 (1998-02-01), Van Gelder et al.
patent: 5763175 (1998-06-01), Brenner
patent: 5837466 (1998-11-01), Lane et al.
patent: 5846719 (1998-12-01), Brenner
patent: 5863722 (1999-01-01), Brenner
patent: 6077673 (2000-06-01), Chenchik et al.
patent: 6235483 (2001-05-01), Wolber et al.
patent: 6238865 (2001-05-01), Huang et al.
patent: 6261770 (2001-07-01), Warthoe
patent: 6558908 (2003-05-01), Wolber et al.
patent: 2001/0026919 (2001-10-01), Chenchik et al.
patent: 2004/0209376 (2004-10-01), Natan et al.
patent: 2005/0158772 (2005-07-01), Lockhart et al.
patent: WO 9727317 (1997-07-01), None
patent: WO 97/31256 (1997-08-01), None
patent: WO 98/24933 (1998-06-01), None
Lombardo et al. Altered brain sodium channel transcript levels in human epilepsy. Molecular Brain Research, vol. 35, Issues 1-2, Jan. 1996, pp. 84-90.
“Population.” Merriam-Webster Online Dictionary. 2004. http://www.merriam-webster.com (Jan. 2, 2004). Two printed pages. Accessed Aug. 14, 2006.
Lost Springs, Wyoming. (Jul. 8, 2006). In Wikipedia, The Free Encyclopedia. Retrieved 15:09, Aug. 14, 2006, from http://en.wikipedia.org/w/index.php?title=Lost—Springs%2C—Wyoming&oldid=62708768. One printed page.
“Group.” Merriam-Webster Online Dictionary. 2004. http://www.merriam-webster.com (Jan. 2, 2004.) Two printed pages. Accessed Aug. 14, 2006.
Zhou et al. New primer strategy improves precision of differential display. BioTechniques (1995) 18(5): 842-844, 846, 848, 850.
“Genisphere—A Datascope Company: 3DNA Submicro Expression Array Detection Kit,” http://www.genisphere.com/pdf/submicro.pdf, boaring a notice “2000, Genisphere, Inc. All rights reserved”.
“3DNA Products: 3DNA Submicro Expression Array Detection Kits,” http://www.genisphere.com/ExpressionArrays.html, bearing a 1998-99 copyright notice.
Ahern. Biochemical, Reagents Kits Offer Scientist Good Return on Investment The Scientist, vol. 9, No. 15. pp. 1-9.
Velculescu et al. Serial Analysis of Gene Expression. Science, vol. 270, pp. 484-487, 1995.

Affiliated with

Also associated with

Rating

Methods and kits for indirect labeling of nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and kits for indirect labeling of nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and kits for indirect labeling of nucleic acids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3957864