Optics: measuring and testing – By dispersed light spectroscopy – Utilizing a spectrometer
Reexamination Certificate
2008-01-15
2008-01-15
Toatley, Jr., Gregory J. (Department: 2877)
Optics: measuring and testing
By dispersed light spectroscopy
Utilizing a spectrometer
C356S317000, C356S318000, C356S332000, C703S002000
Reexamination Certificate
active
10924422
ABSTRACT:
A system and a method for setting a fluorescence spectrum measurement system for microscopy is disclosed. Using illuminating light (3) from at least one laser that emits light of one wavelength, a continuous wavelength region is generated. Dyes are stored, with the pertinent excitation and emission spectra, in a database of a computer system (23). For each dye present in the specimen (15), a band of the illuminating light (3) and a band of the detected light (17) are calculated, the excitation and emission spectra read out from the database being employed. Setting of the calculated band in the illuminating light and in the detected light is performed on the basis of the calculation. Lastly, data acquisition is accomplished with the spectral microscope (100).
REFERENCES:
patent: 3720822 (1973-03-01), Rochester et al.
patent: 5303026 (1994-04-01), Strobl et al.
patent: 5591981 (1997-01-01), Heffelfinger et al.
patent: 5813987 (1998-09-01), Modell et al.
patent: 5828452 (1998-10-01), Gillispie et al.
patent: 5886784 (1999-03-01), Engelhardt
patent: 6069689 (2000-05-01), Zeng et al.
patent: 6097870 (2000-08-01), Ranka et al.
patent: 6166804 (2000-12-01), Potyrailo et al.
patent: 6300639 (2001-10-01), Wiederhoeft
patent: 6556236 (2003-04-01), Swift et al.
patent: 6571118 (2003-05-01), Utzinger et al.
patent: 6584413 (2003-06-01), Keenan et al.
patent: 6690817 (2004-02-01), Cabib et al.
patent: 6738502 (2004-05-01), Coleman et al.
patent: 6766184 (2004-07-01), Utzinger et al.
patent: 6825928 (2004-11-01), Liu et al.
patent: 6870613 (2005-03-01), Tisone et al.
patent: 2001/0041843 (2001-11-01), Modell et al.
patent: 2003/0030801 (2003-02-01), Levenson et al.
patent: 2003/0155494 (2003-08-01), Olschewski
patent: 2004/0036763 (2004-02-01), Swift et al.
patent: 2006/0007439 (2006-01-01), Corcoran
patent: 19829944 (2000-01-01), None
patent: 100 06 800 (2001-08-01), None
patent: 10206979 (2003-08-01), None
patent: 102 27 111 (2003-12-01), None
Warner, I.M., Christian, G.D, Davidson, E.R., Callis, J.B., “Analysis of multicomponent fluorescence data,” Analytical Chemistry (1977), 49(4), 564-73.
Warner, I.M., Christian, G.D, Davidson, E.R., “Quantitative analyses of multicomponent fluorescence data by the methods of least squares and non-negative least sum of errors”, Analytical Chemistry (1977), 49(14), 2155-9.
Vives, MI., Gargallo, R., Tauler, R., “Three-Way Multivariate Curve Resolution Applied to Speciation of Acid-Base and Thermal Unfolding Transaction of an Alternating Polynucleotide”, Biopolymers, vol. 59, 477-488 (2001).
Giglio Bryan J
Houston Eliseeva LLP
Leica Microsystems CMS GmbH
Toatley , Jr. Gregory J.
LandOfFree
Method for separating fluorescence spectra of dyes present... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for separating fluorescence spectra of dyes present..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for separating fluorescence spectra of dyes present... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3956606