Reagent kit of global analysis for protein expression and...

Details Reagent kit of global analysis for protein expression and... Reagent kit of global analysis for protein expression and...

2008-03-04

2008-03-04

Gitomer, Ralph (Department: 1657)

Chemistry: analytical and immunological testing

Including use of radioactive properties

Reexamination Certificate

active

10909294

ABSTRACT:
The present invention provides a reagent kit of global analysis for protein expression and method for qualitative and quantitative proteomic analysis using the same, characterized in which isotope labeling reagent is utilized to modify enzymatically cleaved peptides in normal or perturbed cells and subsequently tandem mass spectrometry is used to identify protein sequence, and at the same time, accurately measure the protein expression level based on variation in signal intensity.

REFERENCES:
Dottavio-Martin et al. Analytical Biochemistry 1978;87:562-565.
Gluck et al. Biochimica et Biophysica Acta 1990;1038:146-151.

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