Animal component free meningococcal polysaccharide...

Details Animal component free meningococcal polysaccharide... Animal component free meningococcal polysaccharide...

2008-07-15

2008-07-15

Lilling, Herbert J (Department: 1657)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S071100, C435S252300, C536S123000

Reexamination Certificate

active

11204562

ABSTRACT:
Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides ofN. meningitidisserotypes A, C, Y and W-135 and is run reproducibly at the 20L scale and can be scaled to 400L or more.

REFERENCES:
patent: 5019502 (1991-05-01), Rienstra et al.
patent: 5314811 (1994-05-01), Lee et al.

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