Microscope for investigating the lifetime of excited states...

Radiant energy – Photocells; circuits and apparatus – Optical or pre-photocell system

Reexamination Certificate

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C250S458100

Reexamination Certificate

active

11106193

ABSTRACT:
A microscope for investigating the lifetime of excited states in a sample has a light source that generates excitation light, and has a detector that receives detected light proceeding from the sample. The light source contains a semiconductor laser which emits pulsed excitation light. An adjusting apparatus is provided for adjusting the pulse repetition rate to the specific lifetime properties of the sample.

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D.W. Piston et al., “Two-photon-excitation fluorescence imaging of three-dimensional calcium-ion activity”, Applied Optics, vol. 33, No. 4, Feb. 1, 1994, pp. 662-669.
D.W. Piston et al., “Time-Resolved Fluorescence imaging and Background Rejection by Two-Photon Excitation in Laser Scanning Microscopy”, SPIE vol. 1640, Time Resolved Laser Spectroscopy in Biochemistry III (1092), pp. 381-389.

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