Method, use and kit for separating albumin from contaminants...

Details Method, use and kit for separating albumin from contaminants... Method, use and kit for separating albumin from contaminants...

2008-04-01

2008-04-01

Mondesi, Robert (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

C530S413000

Reexamination Certificate

active

10514544

ABSTRACT:
The present invention is a method of separating recombinant human serum albumin (rHSA) from low molecular weight contaminants in a liquid, which method comprises the steps of: (a) providing a separation medium, which includes anion-exchanging ligands coupled to a base matrix; and (b) contacting the liquid with the separation medium to adsorb the rHSA to the ligands. In one embodiment, the functional groups of the ligands are weak anion-exchanging groups, preferably secondary amines, and the density of ligands on the base matrix is relatively high.

REFERENCES:
patent: 4093612 (1978-06-01), Travis et al.
patent: 4308254 (1981-12-01), Tayot et al.
patent: 5710253 (1998-01-01), Ohtani et al.
patent: 5849874 (1998-12-01), van der Laken et al.
patent: 6617133 (2003-09-01), Noda et al.
patent: 0 699 687 (1996-03-01), None
patent: WO 02/05959 (2002-01-01), None
Sumi et al. Bioseparation 8: 195-200 (1999). “Purification of Recombinant Human Serum Albumin Efficient purification using STREAMLINE”.
GE Healthcare Instructions 71-5009-64 AC Ion exchange media (DEAE Sepharose Fast Flow), 2005.
Wikipedia web site for Agarose definition, accessed on Internet at noon on Feb. 13, 2006.
Quirk et al., “Production of recombinant human serum albumin fromSaccharomyces cerevisiae”, Biotechnol. Appl. Biochem. 11: 273-287 (1989).

Affiliated with

Also associated with

Rating

Method, use and kit for separating albumin from contaminants... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method, use and kit for separating albumin from contaminants..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method, use and kit for separating albumin from contaminants... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3949120