Enzyme/immunofluorescent assay for anti-Epstein-Barr Virus antib

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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Details

435 5, 435 28, G01N 3353, C12Q 170, C12Q 128

Patent

active

046070087

ABSTRACT:
A method for the determination of anti-Epstein-Barr Virus antibodies in a test sample comprises contacting a substrate for the anti-Epstein-Barr Virus antibodies with sample; treating the contacted substrate with labeled antihuman Ig antibody selected from (a) a mixture comprising enzyme labeled antihuman Ig antibody and fluorescent labeled antihuman Ig antibody, (b) antihuman Ig antibodies labeled with an enzyme and a fluorescent label, (c) fluorescent labeled antihuman Ig antibody to which enzyme labeled antibody against the animal species from which the antibody used in the fluorescent labeled antibody was derived is subsequently added, and (d) enzyme labeled antihuman Ig antibody to which fluorescent labeled antibody against the animal species from which the antibody used in the enzyme labeled antibody was derived is subsequently added; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening for anti-Epstein-Barr Virus antibodies for the diagnosis of Infectious Mononucleosis.

REFERENCES:
patent: 4144031 (1979-03-01), Acevedo et al.
patent: 4228127 (1980-10-01), Acevedo et al.
patent: 4385126 (1983-05-01), Chen et al.
patent: 4487830 (1984-12-01), Coates et al.

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