Mutant carotenoid ketolases

Details Mutant carotenoid ketolases Mutant carotenoid ketolases

2008-07-01

2008-07-01

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S325000, C435S252300, C435S252310, C435S252320, C435S252330, C435S252340, C435S252350, C435S069100, C435S410000, C435S254110, C435S257200, C435S254200, C435S254300, C435S254600, C435S254210, C435S254230, C435S254220, C435S067000, C435S320100, C536S023200, C530S350000

Reexamination Certificate

active

11393082

ABSTRACT:
CrtW carotenoid ketolases are provided that are useful for the production of astaxanthin and other cyclic ketocarotenoids. The mutant ketolase genes of the present invention encode polypeptides characterized by an improvement in astaxanthin synthesis activity when converting cyclic hydroxylated carotenoid intermediates into astaxanthin. Expression of the mutant carotenoid ketolases in heterologous hosts enabled increased production of astaxanthin relative to theSphingomonas melonisDC18 CrtW.

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