Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1994-11-02
1997-06-03
Ziska, Suzanne E.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435378, 435373, 435375, 435395, C12N 500
Patent
active
056353863
ABSTRACT:
Methods, including culture media conditions, which provide for in vitro human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells and/or a method for assaying the effect of a substance or condition on a human hematopoietic cell population, and/or depleting the malignant cell or T-cell and B-cell content of a human hematopoietic cell population are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally, growth factors are added to the culture medium. The disclosed culture conditions afford improved methods for bone marrow transplantation.
REFERENCES:
patent: 4481946 (1984-11-01), Altshuler et al.
patent: 4486188 (1984-12-01), Altshuler et al.
patent: 4963489 (1990-10-01), Naughton et al.
patent: 5032407 (1991-07-01), Wagner et al.
patent: 5032508 (1991-07-01), Naughton
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5199942 (1993-04-01), Gillis
patent: 5399493 (1995-03-01), Emerson
Anderson Science 226: 401, 1984.
Dester Nature 309 97, 1984.
Dester Nature 309 97, 1984.
Anderson Science 226: 401, 1984.
Gail K. Naughton, et al.; Journal of Cellular Biochemistry; Hematopoeisis on Nylon Mesh Microenvironments; 19th Annual Meeting (1990).
Jerry Caldwell, et al.; Biotechnology Progress; Influence of Medium Exchange Schedules on Metabolic, Growth, and GM-CSF Secretion Rates of Genetically Engineered NIH-3T3 Cells; vol. 7; pp. 1-8; (1991).
Jerry Caldwell, et al.; Journal of Cellular Physiology; Culture Perfusion Schedules Influence the Metabolic Activity and Granulocyte-Macrophage Colony-Stimulating Factor Production Rates of Human Bone Marrow Stromal Cells; vol. 147, No. 2; pp. 344-353; (1991).
Richard M. Schwartz, et al.; BLOOD; In vitro Myelopoiesis Stimulated by Rapid Medium Exchange and Supplementation with Hematopoietic Growth Factors; vol. 78, No. 12; pp. 3155-3161; (1991).
Richard M. Schwartz, et al.; Proceedings of the National Academy of Sciences; Rapid Medium Perfusion Rate Significantly Increases the Productivity and Longevity of Human Bone Marrow Cultures; vol. 88, No. 15; pp. 6760-6764; (1991).
"Expansion of Human Bone Marrow Progenitor Cells in a High Cell Density Continuous Perfusion System"; Bernhard O. Palsson, et al; Bio/Technology, vol. 11, Mar. 1993; pp. 368-372.
"Expansion of Primitive Human Hematopoietic Progenitors in a Perfusion Bioreactor System with IL-3, IL-6, and Stem Cell Factor"; Manfred R. Koller, et al; Bio/Technology, vol. 11; Mar., 1993; pp. 358-363.
"Characterization and Partial Purification of Human Marrow Cells Capable of Initiating Long-Term Hematopoiesis in vitro"; Heather J. Sutherland, et al; Blood; vol. 74, No. 5, Oct., 1989; pp. 1563-1570.
"Functional Characterization of Individual Human Hematopoietic Stem Cells Cultured at Limiting Dilution of Supportive Marrow Stromal Layers"; Heather J. Sutherland, et al; Proc. Natl. Acad. Sci. USA; vol. 87, May, 1990; pp. 3584-3588.
"The Human Hematopoietic Stem Cell in Vitro and In Vivo"; C.J. Eaves, et al; Blood Cells; 18; 1992; pp. 301-307.
"Alternative Mechanisms With and Without Steel Factor Support Primitive Human Hematopoiesis"; H.J. Sutherland, et al; Blood; vol. 81, No. 6, Mar. 15, 1993; pp. 1464-1470.
"Human Recombinant Granulocyte-Macrophage Colony-Stimulating Factor: A Multilineage Hematopoietin"; Colin A. Sieff, et al; Science, vol. 230; Dec., 1985; pp. 1171-1173.
"Use of Limiting-Dilution Type Long-Term Marrow Cultures in Frequency Analysis of Marrow-Repopulating and Spleen Colony-Forming Hematopoietic Stem Cells in the Mouse"; Rob E. Ploemacher, et al; Blood, vol. 78, No. 10; Nov. 15, 1991; pp. 2527-2533.
"Evaluation of Hematopoiesis in Long-Term Bone Marrow Culture: Comparison of Species Differences"; C.E. Eastment, et al; Long-Term Bone Marrow Culture; 1984; pp. 97-118.
"Bone Marrow Stromal Fibroblasts Secrete Interleukin-6 and Granulocyte-Macrophage Colony-Stimulating Factor in the Absence of Inflammatory Stimulation: Demonstration by Serum-Free Bioassay, Enzyme-Linked Immunosorbent Assay, and Reverse Transcriptase Polymerase Chain Reaction"; Susan C. Guba, et al; Blood, vol. 80, No. 5; Sep. 1, 1992; pp. 1190-1198.
"Formation of haematopietic microenvironment and haematopoietic stem cells from single human bone marrow stem cells"; Shiang Huang, et al; Nature, vol. 360; Dec. 24-31, 1992; pp. 745-749.
"Primitive hemopoietic stem cells: direct assay of most productive populations by competitive repopulation with simple binomial, correlation and covariance calculations"; David E. Harrison, et al; Experimental Hematology, 21; 1993; pp. 206-219.
"Methodology of Long-Term Culture of Human Hematopoietic Cells"; Connie J. Eaves, et al; J. Tiss. Cult. Meth., 13; 1991; pp. 66-62.
"Expression of Human Adenosine Deaminase in Nonhuman Primates After Retrovirus-Mediated Gene Transfer"; Philip W. Kantoff, et al; J. Exp. Med., vol. 166; Jul., 1987; pp. 219-234.
"Beneficial Effects of Reduced Oxygen Tension and Perfusion in Long-Term Hematopoietic Cultures"; Manfed R. Koller, et al; Biochemical Engineering VII, vol. 665 of the Annals of the New York Academy of Sciences; Oct. 13, 1992; pp. 105-116.
"Effects of Synergistic Cytokine Combinations, Low Oxygen, and Irradiated Stroma on the Expansion of Human Cord Blood Progenitors"; Manfred R. Koller, et al; Blood, vol. 80, No. 2; Jul. 15, 1992; pp. 403-411.
"Prospects for Human Gene Therapy"; W. French Anderson; Science, vol. 226; Oct., 1984; pp. 401-409.
"Reduced Oxygen Tension Increases Hematopoiesis in Long-term Culture of Human Stem and Progenitor Cells from Cord Blood and Bone Marrow"; Manfred R. Koller, et al; Experimental Hematology, 20; 1992; pp. 264-270.
"Recent Modifications of Technique in Human Long-Term Bone Marrow Cultures"; Joel S. Greenberger; Long-Term Bone Marrow Culture; 1984; pp. 119-131.
"The Message in the Medium"; T.M. Dexter; Nature, vol. 309, No. 28; Jun., 1984; pp. 746-747.
"Long-term culture of human bone marrow cells"; Suzanne Gartner, et al; Proc. Natl. Acad. Sci. USA, vol. 77, No. 8; Aug., 1980; pp. 4756-4759.
"Granuloerythropoietic Colonies in Human Bone Marrow, Peripheral Blood, and Cord Blood", A.A. Fauser, et al; Blood, vol. 52, No. 6; Dec., 1978; pp. 1243-1248.
"Induction of Colonies of Hemoglobin-Synthesizing Cells by Erythropoietin In Vitro"; John R. Stephenson, et al; Proc. Nat. Acad. Sci. USA, vol. 68, No. 7; Jul., 1971; pp. 1542-1546.
"Retrovirus-mediated transfer and expression of drug resistance genes in human haematopoietic progenitor cells"; Randy A. Hock, et al; Nature, vol. 230; Mar. 20, 1986; pp. 275-277.
"Canine Model for Gene Therapy: Inefficient Gene Expression in Dogs Reconstituted With Autologous Marrow Infected With Retroviral Vectors"; Richard B. Stead, et al; Blood, vol. 71, No. 3; Mar., 1988; pp. 742-747.
"The Basic Science of Gene Therapy"; Richard C. Mulligan; Science, vol. 260; May 14, 1993; pp. 926-932.
"Mammalian Cell Culture; engineering principles and scale-up"; M.W. Glacken, et al; Trends in Biotechnology, vol. 1, No. 4; 1983; pp. 102-108.
"A Cell Culture Model for T Lymphocyte Clonal Anergy"; Ronald H. Schwartz; Science, vol. 248; Jun. 15, 1990; pp. 1349-1356.
Cell, vol. 47, pp. 3-10, Oct. 10, 1986, Yu-Chung Yang, et al., "Human IL-3 (Multi-CSf): Identification By Expression Cloning of a Novel Hematopoietic Growth Factor Related to Murine IL-3".
The Journal of Antibiotics, vol. XXXIII, No. 6, Jun. 1980, pp. 653-662, M. Ishizuka, et al., "Mitogenic Effect of Bestatin on Lymphocytes".
The Journal of Immunology, vol. 121, No. 5, Nov. 1978, pp. 1951-1955, S.A. Rosenberg, et al., "In Vitro Growth of Murine T Cells. II. Growth of In Vitro Sensitized Cells
Armstrong R. Douglas
Clarke Michael F.
Emerson Stephen G.
Palsson Bernhard O.
The Regents of the University of Michigan
Ziska Suzanne E.
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