Methods of obtaining a DNA sequence for integration site indepen

Details Methods of obtaining a DNA sequence for integration site indepen Methods of obtaining a DNA sequence for integration site indepen

1995-06-07

1997-06-03

Rories, Charles C. P.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435 911, 435 914, 4351723, 800 2, 800DIG1, 800DIG2, 536 241, C12N 1510, C12N 1585, C12N 1563, C12N 516

Patent

active

056353553

ABSTRACT:
Vectors for the integration of a gene into the genetic material of a mammalian host cell such that the gene may be expressed by the host cell comprise a promoter and the gene and include a dominant activator sequence capable of eliciting host cell-type restricted, integration site independent, copy number dependent expression of the gene.

REFERENCES:
Chao et al., "The Regulated Expression of .beta.-Globin Genes Introduced into Mouse Erythroleukemia Cells", Cell, vol. 32, pp. 483-493, (1983).
Gross et al., "Nuclease Hypersensitive Sites in Chromatin", Ann. Rev. Biochem., vol. 57, pp. 159-197, (1988).
Grosschedl et al., "Introduction of a .mu. Immunoglobulin Gene into the Mouse Germ Line: Specific Expression in Lymphoid Cells and Synthesis of Function Antibody", Cell, vol. 38, pp. 647-658 (Oct. 1984).
Kollias et al., "Regulated Expression of Human .sup.A .gamma., .beta.-, and Hybrid .gamma..beta.-Globin Genes in Transgenic Mice: Manipulation of the Developmental Expression Patterns", Cell, vol. 46, pp. 89-94, (Jul. 4, 1986).
Lang et al., "The structure of the Human CD2 gene and its expression in transgenic mice", The EMBO Journal, vol. 7, No. 6, pp. 1675-1682, (Jun., 1988).
Li et al., "Nucleotide Sequence of 16-Kilobase Pairs of DNA 5 ' to the Human .epsilon.-Globin", The Journal of Biology and Chemistry, pp. 14901-14910.
Magram et al., "Developmental regulation of a cloned adult .beta.-globin gene in transgenic mice", Nature, vol. 315, pp. 338-340, (May, 1995).
Marks et al., "Erythroleukemic Differentiation", Ann. Rev. Biochem., vol. 47, pp. 419-448, (1978).
Tuan et al., "The .beta.-like-globin gene domain in human erythroid cells", Proc. Natl. Acad. Sci. USA, vol. 82, pp. 6384-6388, (Oct. 1985).

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