Method for detecting sensitivity to acetylcholine esterase...

Details Method for detecting sensitivity to acetylcholine esterase... Method for detecting sensitivity to acetylcholine esterase...

2007-08-07

2007-08-07

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S183000

Reexamination Certificate

active

10320710

ABSTRACT:
A method for checking for sensitivity of acetylcholine esterase in insects using a single insect, includes homogenizing a single insect in aqueous, neutral to acid pH phosphate buffer, providing a solution of acetylcholine iodide in neutral to acid pH phosphate buffered water-miscible organic solvent, providing a solution of 5,5-dithio-bis-(2-nitrobenzoic acid) in the buffer, and providing a solution of propoxur in the buffer, dropping the insect homogenisate into the wells of an assay plate, dropping the acetylcholine iodide and 5,5-dithio-bis-(2-nitrobenzoic acid) solutions into some of the wells and 5,5-dithio-bis-(2-nitrobenzoic acid) propoxur solutions into the other wells and checking for a difference in the yellow coloration between the samples in the two sets of cells.

REFERENCES:
patent: 5686237 (1997-11-01), Al-Bayati
CDC, “Evaluating Mosquitoes for Insecticide Resistance”(Web-Based Instruction), www.cdc.gov
cidod/wbt/resistance/assay/microplate/step—2.htm, (May 2002).
Arufe M. Oxidation of Cholinesterase Inhibiting Pesticides. Biochemical Education 28(3)174-177, 2000.

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