Method and apparatus for preparing monolayers of cells

Details Method and apparatus for preparing monolayers of cells Method and apparatus for preparing monolayers of cells

2007-08-14

2007-08-14

Lankford, Jr., Leon B (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S001300, C435S040510, C435S283100, C435S307100, C435S176000, C435S173300, C435S182000, C435S374000

Reexamination Certificate

active

10172473

ABSTRACT:
The invention concerns an apparatus for preparing monolayers of cells. The apparatus comprises a container (6) for a cryosubstitution system and an insert (1) for the container, the insert (1) having a surface (1a) and a plurality of orifices (3). An SCS (2) together with a cellular monolayer (20) is insertable into each of the orifices (3). The SCS (2) is thus arranged perpendicular to the surface (1a) of the insert (1).

REFERENCES:
patent: 4306425 (1981-12-01), Sitte et al.
patent: 29 44 464 (1981-05-01), None
patent: 37 12 531 (1988-05-01), None
Hernandez-Verdun et al. Biology of the Cell 72 pp. 121-132 1991.
“Reichert AFS, Automatic Freeze-Substitution-System, according to Sitte and Edelmann” Leica/Reichert Division Brochure 1993.

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