Primers useful in polymerase chain reaction for the...

Details Primers useful in polymerase chain reaction for the... Primers useful in polymerase chain reaction for the...

2007-08-21

2007-08-21

Strzelecka, Teresa E. (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S024320, C435S006120, C435S091200

Reexamination Certificate

active

10261467

ABSTRACT:
The invention provides primers designed on base of the difference of sequences among staphylococcal enterotoxin subtypes, i.e., C1, C2and C3. The invention also provides a PCR method for detecting subtypes of staphylococcal enterotoxin type C by using the above-mentioned primers. The invention relates also to DNA probes useful for the identification of subtypes C1, C2and C3of staphylococcal enterotoxin type C in various food and clinical samples. The primers of the invention comprises following sequences:ENTC1 5′-ACAGA GTTAT TAAAT GAAGG-3′;ENTC2 5′-GTATC AGCAA CTAAA GTTAT-3′;ENTC3 5′-AAGAG ATTAT TTATT TCACGT-3′;ENTCR 5′-ATCAT ACCAA AAAGT ATTGC-3′.

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