Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2007-06-26
2007-06-26
Winkler, Ulrike (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C435S006120, C435S007100, C435S007210, C435S320100, C536S023720
Reexamination Certificate
active
11233461
ABSTRACT:
The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.
Huang Wei
Parkin Neil T.
Petropoulos Christos J.
Whitcomb Jeanette
Jones Day
Monogram Biosciences, Inc.
Winkler Ulrike
LandOfFree
Methods of evaluating cell surface receptor binding of a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of evaluating cell surface receptor binding of a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of evaluating cell surface receptor binding of a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3887080