Detection of plant pathogenic fungi

Details Detection of plant pathogenic fungi Detection of plant pathogenic fungi

1996-05-23

1998-08-11

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 536 2432, 536 2433, 935 8, 935 77, 935 78, C12Q 168

Patent

active

057926115

ABSTRACT:
Root rots are serious conifer nursery diseases. At present, seedlings are inspected visually to determine whether they show symptoms of disease. Cylindrocarpon destructans and Cylindrocarpon floridanum are two important causal agents of root rots. A substantially more efficient and reliable method of detecting the presence of these fungi directly from infected tissue involves the use of a set of DNA primers which can be used in conjunction with the polymerase chain reaction (PCR) to create species specific probes for detecting species of each fungi.

REFERENCES:
patent: 5324632 (1994-06-01), Weisburg et al.
patent: 5434048 (1995-07-01), Simon et al.
Brown et al. (1993) FEMS Microbiology Letters. vol. 108, pp. 117-120.
Accession No. U36443, Dumas, M. et al. (17 Oct. 1995) EMBL/GenBank/DDBJ Database. Cylindrocladium Floridanum rDNA internal transcribed spacer.

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