Method for efficiently producing transgenic plant using...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters plant part growth

Reexamination Certificate

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C800S282000, C800S288000, C800S294000, C800S300000, C800S323000, C435S193000, C435S195000, C435S320100, C435S456000, C435S470000

Reexamination Certificate

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10626609

ABSTRACT:
A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme which synthesizes auxin from an auxin precursor; (B) culturing the plant cell into which the genes are introduced by the vector, in the presence of an auxin precursor and/or an analogue thereof to thereby prepare a redifferentiated tissue, and detecting and selecting the redifferentiated tissues; and (C) culturing the redifferentiated tissue selected in (B) to redifferentiate a plant individual, and a vector for gene introduction into a plant, which comprises: a desired gene, and a selectable marker gene comprising an indoleacetamide hydrolase, iaaH, gene and an isopentenyl transferase, ipt, gene and being free of an tryptophan monooxygenase, iaaM, gene.

REFERENCES:
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