Methods for detecting malaria parasites and reagent kits...

Optics: measuring and testing – With sample preparation

Reexamination Certificate

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C356S073000, C435S002000, C435S026000, C435S372000, C514S614000, C204S547000

Reexamination Certificate

active

10678536

ABSTRACT:
Methods for detecting a malaria parasite are described that include: releasing the malaria parasite by allowing an erythrocyte in a specimen to undergo hemolysis; staining the released malaria parasite with a fluorescent dye to prepare a measurement sample; detecting optical information from the measurement sample; and distinguishing the malaria parasite based on the optical information obtained. Apparatuses and reagent kits are also described.

REFERENCES:
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Saito-Ito, A. et al. 2001. A rapid, simple and sensitive flow cytometric system for detection ofPlasmodium falciparum. Parasitology International 50: 249-257.
van Vianen, P.H. et al. 1993. Flow cytometric screening of blood samples for malaria parasites. Cytometry 14: 276-280.
Hawley's Condensed Chemical Dictionary (14th Edition, 2002, John Wiley and Sons, Inc.).
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Hare, J. Donald; and Bahler, David W., “Analysis ofPlasmodium falciparumGrowth in Culture Using Acridine Orange and Flow Cytometry”,The Journal of Histochemistry and Cytochemistry, 1986, vol. 34, No. 2, pp. 215-220.
Bianco, A. E.; Battye, F. L.; and Brown , G. V., “Plasmodium falciparum: Rapid Quantification of Parasitemia in Fixed Malaria Cultures by Flow Cytometry”,Experimental Parasitology, 1986, vol. 62, pp. 275-282.
van Vianen, Philip H.; van Engen, Anneloes; Thaithong, Sodsri; van der Keur, Maarten; Tanke, Hans J.; van der Kaay, Hugo J.; Mons, Barend; and Janse, Chris J., “Flow Cytometric Screening of Blood Samples for Malaria Parasites”,Cytometry, 1993, vol. 14, pp. 276-280.
Saito-Ito, Atsuko; Akai, Yasumasa; He, Shenyi; Kimura, Mikio; and Kawabata, Masato, “A rapid, simple and sensitive flow cytometric system for detection ofPlasmodium falciparum”, Parasitology International, 2001, vol. 50, pp. 249-257.

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