Radiant energy – Luminophor irradiation – Methods
Patent
1994-12-28
1996-06-04
Fields, Carolyn E.
Radiant energy
Luminophor irradiation
Methods
G01N 2164
Patent
active
055235735
ABSTRACT:
A method for the two-photon excitation of long-lived fluorescent or phosphorescent dyes with long low-power pulses. In the method time-resolved detection is used to detect long-lived dyes. In the method long-lived dyes are excited to the excited state with light pulses of long duration via double-photon absorption. In the two-photon absorption process the chromophor of the dye molecule is excited through the summation of the energies of two or more photons when they are simultaneously absorbed. As the method is based on the use of pulses of long duration and on time-resolved detection the peak power of the pulses may be kept low. Long pulses of low power can be produced with a great variety of low-power light sources. The light source may in this case be e.g. a semiconductor laser. The excitation technique according to the invention may exploit the doubling or multiplication of the excitation wavelength or the nonlinearity of the two-photon absorption process. The method may be used for the detection of the fluorescence or phosphorescence from long-lived dyes for the determination of biospecific binding in applications of analytical or microscopic methods.
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patent: 5034613 (1991-07-01), Denk et al.
Warner L. Peticolas, et al., Physical Review Letters, 15 Jan. 1963, vol. 10, No. 2, pp. 43-45.
M. J. Wirth et al., Anal. Chem, 1990, vol. 62, No. 9, May 1, 1990, pp. 973-976.
Winfried Denk, et al., Science, vol. 248, 6 Apr. 1990, pp. 73-76.
Hanninen Pekka
Soini Erkki
Fields Carolyn E.
Glick Edward J.
Kubovcik Ronald J.
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