Streptomyces avermitilis gene directing the ratio of B2:B1...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C435S253500, C435S007100, C435S069100, C530S350000, C530S300000, C514S002600, C514S012200

Reexamination Certificate

active

11011960

ABSTRACT:
The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures ofS. avermitilis. The present invention further relates to vectors, host cells, and mutant strains ofS. avermitilisin which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

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Ikeda et al., Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin inStreptomyces avermitilis. PNAS (1999) 96: 9509-9514.
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