Method for controlling ratio of proteins/lipids in crop seeds

Details Method for controlling ratio of proteins/lipids in crop seeds Method for controlling ratio of proteins/lipids in crop seeds

2007-02-13

2007-02-13

McElwain, Elizabeth F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

C800S298000

Reexamination Certificate

active

10142111

ABSTRACT:
A method for altering the ratio of proteins/lipids in crop seeds utilizing the antisense gene, comprising the steps of: (1) cloning the gene for the phosphoenopyruvate carboxylase or acetyl-CoA carboxylase, or the fragment thereof; (2) constructing the corresponding antigene; (3) introducing the said antigene into the cells of the rapeseeds, so that the activity of the phosphoenopyruvate carboxylase or the acetyl-CoA carboxylase being important for the protein synthesis is changed, the flow direction of the common substrate, PEP, can be controlled, so that the proteins/lipids in the seeds is altered. The application can be used to alter the proteins/lipids in the crop seeds.

REFERENCES:
patent: 5530192 (1996-06-01), Murase et al.
patent: 5914449 (1999-06-01), Murase et al.
patent: 95-143887 (1995-06-01), None
patent: WO 92/04456 (1992-03-01), None
Gehlen et al, Plant Molecular Biology 32: 831-848, 1996.
Sugimoto, Toshio, et al. “Phosphoenolpyruvate Carboxylase Level in Soybean Seed Highly Correlates to its Contents of Protein and Lipid”,Agri. Biol. Chem.,53 (3); pp. 885-887 (1989).

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