Methods for generating double stranded DNA comprising a...

Details Methods for generating double stranded DNA comprising a... Methods for generating double stranded DNA comprising a...

2007-02-13

2007-02-13

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

C435S006120, C536S023100, C536S024300

Reexamination Certificate

active

10386623

ABSTRACT:
Methods of recombining nucleic acids are provided. In particular, methods for the production of partially double stranded nucleic acids comprising a 3′ overhang from an RNA target and use in methods of recombining polynucleotides is described. These methods do not require thermocycling. The present invention also provides methods of recombining and selection which allow for identification of proteins comprising improved or desired characteristics.

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