Method for isolating and purifying transferrin and lactoferrin r

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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530400, 530350, 530395, 530413, C07K 1514, A61K 3902

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active

051417430

ABSTRACT:
The present invention relates to a method for isolating and purifying transferrin and lactoferrin receptor proteins from bacterial pathogens by affinity chromatography and to vaccine antigens containing the purified receptor proteins.

REFERENCES:
patent: 4434156 (1984-02-01), Trowbridge
patent: 4447547 (1984-05-01), Allen et al.
Schryvers, A. B. et al., 1988, Mol. Microbiol. 2(2): 281-288 (abstract).
Lee et al., 1988, Mol. Microbiol. 2(6): 827-829 (abstract).
Schryvers et al., "Comparative Analysis of the Transferrin and Lactoferrin Binding Proteins in the Family Neisseriaceae", Can J. Microbiol., vol. 35, 1989, pp. 409-415.
Lee et al., "Specificity of the Lactoferrin and Transferrin Receptors in Neisseria Gonorrhoeae", Molecular Microbiology, (1988) 2(6), 827-829.
Abstract, Ala'Aldeen, D. A., Heather A. Davies, Wall, R. A., and Borriello, S. P., "Interaction Between Iron Regulated Outer Membrane Proteins of Neisseria Meningitidis and Human Transferrin Binding Activity".
Selected Pages from the Thesis of Leigh J. Morris, 1988, at the University of Calgary "Meningococcal Receptors for Transferrins".
Identification and Characterization of the Human Lactoferrin-Binding Protein from Neisseria Meningitidis, Schryvers and Morris, Infection and Immunity, May 1988, pp. 1144-1149.
Characterization of the Human Transferrin and Lactoferrin Receptors in Haemophilus Influenzae, Schryvers, Molecular Microbiology (1988) 2(4), pp. 467-472.
Identification of the Transferrin-- and Lactoferrin-Binding Proteins in Haemophilus Influenzae, Schryvers, J. Med. Microbiol., vol. 29, (1989), pp. 121-130.
Comparison of the Abilities of Different Protein Sources of Iron to Enhance Neisseria Meningitidis Infection in Mice, Schryvers and Gonzalez, Infection and Immunity, Aug. 1989, pp. 2425-2429.

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