DNA probes specific for hemolytic listeria

Details DNA probes specific for hemolytic listeria DNA probes specific for hemolytic listeria

1993-10-01

1996-06-04

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2432, C12Q 168, C12P 1934, C07H 2104

Patent

active

055232051

ABSTRACT:
DNA probes are capable of hybridizing to a portion of the genome of pathogenic Listeria monocytogenes, but do not hybridize to genomes of other Listeria species and other hemolytic bacteria. These probes are useful to identify food sources infected with Listeria monocytogenes and to distinguish these food sources from those infected with non-pathogenic Listeria species. In addition, methods for the detection of pathogenic Listeria in samples using the disclosed probes are provided. A method of detection using PCR amplification with primers specific for L. monocytogenes DNA is also disclosed.

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