Method and device employing time-delayed integration for detecti

Details Method and device employing time-delayed integration for detecti Method and device employing time-delayed integration for detecti

1990-11-16

1992-08-25

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 356344, 2101982, 210656, 73 6152, G01N 2726

Patent

active

051416093

ABSTRACT:
This invention relates to a detection method and apparatus useful in capillary electrophoresis and capillary chromatography that employs an array of solid state detector such as a charge-coupled device operating in the time-delayed integration mode which allows more exposure time of the moving analyte zones. The CCD is synchronized so that after a normal exposure of the CCD, the charge information in every row of the CCD is shifted toward one end of the CCD and the charge/signal information in the last row is quantified. Applying the CCD and the time-delayed integration method in effect increases the effective sampling volume of the flow cell without introducing band broadening. Use of the CCD as a fluorescence detection in capillary electrophoresis separations allows analytes to be differentiated both in migration time and fluorescence emission, yielding detection limits for fluorophores in the 1-8.times.10.sup.-20 mole range.

REFERENCES:
patent: 4559695 (1985-12-01), Baker
patent: 4874492 (1989-10-01), MacKay
patent: 4904366 (1990-02-01), Tokita
Cheng et al., "Charge-Coupled Device Fluorescence Detection for Capillary-Zone Electrophoresis (CCD-CZE)", Applied Spectroscopy, vol. 44, No. 5, (1990), pp. 755-765.

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