Use of alpha factor sequences in yeast expression systems

Details Use of alpha factor sequences in yeast expression systems Use of alpha factor sequences in yeast expression systems

2007-04-03

2007-04-03

Wax, Robert A. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S069100, C435S069600, C435S069900, C435S254200, C435S320100

Reexamination Certificate

active

08448946

ABSTRACT:
The isolation of the yeast α-factor genes is described. The promoter and signal peptide portions are isolated and joined to DNA coding for proteins heterologous to yeast in a plasmid which is used to transform yeast cells. The yeast expresses the heterologous DNA and processes and secretes the heterologous protein.

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