Phenethanolamine-derived haptens, immunogens, antibodies and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007920, C435S007930, C435S975000, C436S544000, C436S546000, C436S056000, C530S402000, C530S403000, C530S388900, C530S389800

Reexamination Certificate

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11271282

ABSTRACT:
The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E:in which, in formula I, Z1is a crosslinker and Z2is H and, in formula II, Z1is H and Z2is a crosslinker.

REFERENCES:
patent: 6274334 (2001-08-01), Shelver et al.
Elliot, C.T. et al., “Screening and Confirmatory Determination of Ractopamine Residues in Calves Treated with Growth Promoting Doses of the β-Agonist”,Analyst, May 1998, 123, 1103-1107.
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Shelver, W.L. et al., “Production and Characterization of Monoclonal Antibody against the β-Adrenergic Agonist Ractopamine”,J. Agric. Food Chem, 2000, 48, 4020-4026.
Shelver, W.L. et al., “Development of an Immunoassay for the β-Adrenergic Agonist Ractopamine”,Journal of Immunoassay, 2000, 21(1), 1-23.
Shelver, W.L. et al., “Determination of Ractopamine in cattle and Sheep Urine Samples Using an Optical Biosensor Analysis: Comparative Study with HPLC and ELISA”,J. Agric. Food Chem, 2003, 51, 3715-3721.
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Haasnoot, W. et al., “Immunofiltration as Sample Cleanup for the Immunochemical Detection of β-Agonists in Urine”,Analyst, The Royal Society of Chemistry, 2002, 127, 87-92.
Madding, G.D., “Synthesis of Tritium Labeled Isoxsuprine Hydrochloride”,Journal of Labeled Compounds, 1971, 7(4), 393-397.
Ren, J.Z. et al., “Synthesis of Ritodrine”,Zhongguo Yiyao Gongye Zazhi, 2000, 31(6), 241-242.
Shelver, W.L. et al., “Application of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Determination of Ractopamine in Incurred Samples from Food Animals”,J. Aric. Food Chem., 2002, 50, 2742-2747.
Terando, N.H., “Synthesis of 14C-Radiolabeled Ractopamine Hydrochloride”,Journal of Labelled Compounds and Radiopharmaceuticals, 1992, 31(9), 651-658.

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