Fluorescence intensity compensation method and device

Details Fluorescence intensity compensation method and device Fluorescence intensity compensation method and device

1985-07-15

1986-11-11

Fields, Carolyn E.

Radiant energy

Luminophor irradiation

2504591, 356317, G01N 2164

Patent

active

046224688

ABSTRACT:
Variations in the intensity of a light source and the sensitivity of a fluorescence detector photo-multiplier tube are simultaneously compensated by measuring the intensity (I.sub.LS1) of the light source at the sample excitation frequency with a silicon detector, measuring the intensity (I.sub.LS2) of the light source at the emission frequency with another silicon detector, measuring with the photo-multiplier detector the intensity (I.sub.S) of the sample's fluorescence and the intensity (I.sub.LS3) of the light source at the emission frequency of the sample and then calculating a value F as representative of the sample's fluorescence according to the formula: ##EQU1##

REFERENCES:
patent: 3854050 (1974-12-01), Peterson et al.
patent: 3918812 (1975-11-01), Holm
patent: 4198567 (1980-04-01), Eneroth et al.
patent: 4516856 (1985-05-01), Popelka

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