Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2007-09-18
2007-09-18
Bragdon, Kathleen Kerr (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C930S010000, C536S023100, C530S857000
Reexamination Certificate
active
10501098
ABSTRACT:
Described is a nucleic acid coding for a protein called “cyplasin” that shows a preferential toxicity to autonomously growing mammalian cells. Cell death induced by this protein differs from both apoptosis and necrosis. An intracellular cell death which occurs when recombinantly preparing cyplasin in cell cultures can be avoided by removal of the secretion signal in the cyplasin sequence. This modification makes it possible to express the cyplasin in a mammalian cell culture which is preferable with regard to the glycosylation pattern of the obtained protein. Thus, the present invention also relates to a method of recombinantly producing a protein in eukaryotic cells, preferably mammalian cells, which is cytotoxic for said cells when applied externally.
REFERENCES:
Takamatsu et al. Molecular Cloning of the Defense Factor in the Albumen Gland of the Sea HareAplysia kurodai. FEBS Lett. 1995. vol. 377, pp. 373-376.
Edmondson et al. Structure and Mechanism of Monoamine Oxidase. Current Medicinal Chemistry. 2004. vol. 11, pp. 1983-1993.
Bowie et al., Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions. Science. 1990. vol. 247, pp. 1306-1310.
Wells, J.A., Additivity of Mutational Effects in Proteins. Biochemistry. 1990, vol. 29, No. 37, pp. 8509-8517.
Kamiya et al. Aplysianin-A, an Antibacterial and Antineoplastic Glycoprotein in the Albumen Gland of a Sea Hare,Aplysia kurodai. Experientia. 1986. Vo. 42, pp. 1065-1067.
Appendix A—Sequence alignment of SEQ ID No. 1 (20-558) and Takamatsu et al. No date.
Yamazaki, M., Antitumor and antimicrobial glycoproteins from sea hares. Comp. Biochem. Physiol C. Jun. 1993 vol. 105, No. 2, pp. 141-146.
Appendix B—Sequence alignment of SEQ ID No. 1 to Petzelt AJ304802. No date.
Petzelt, C.P. “Aplysia punctata, mRNA for cyplasin L (ek431 gene)” Database accession No. AJ304802, XP002240886, cited in application, Dec. 21, 2000.
Petzelt, et al., “Cyplasin kills preferentially autonomous cycling cells”, Cell Biology International, Academic Press, GB, vol. 25, No. 2, 2001, p. A23, XP001002719, ISSN: 1065-6995, asbtract.
Suzuki, et al., “Lethal effect of the expression of a killer gene SMK1inSaccharomyces cerevisiae”, Protein Engineering, England Feb. 2000, vol. 13 No. 2 pp. 73-76, XP002240884, ISSN: 0269-2139, p. 74, left-hand column, paragraph 3.
Petzelt, et al., “Cyplasin Cyplasin of the Sea Hare,Aplysia punctata, cDNA Cloning, and Expression of Bioreactive Recombinants in Insect Cells”, Neoplasia (New York), vol. 4, No. 1, Jan. 2002 pp. 4-59, XP002240885, ISSN: 1522-8002, the whole document.
Bragdon Kathleen Kerr
Cyplasin Biomedical, Ltd.
Hultquist Steven J.
Intellectual Property / Technology Law
Noakes Suzanne M.
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