Method for rapid thermal cycling of biological samples

Details Method for rapid thermal cycling of biological samples Method for rapid thermal cycling of biological samples

2007-07-03

2007-07-03

Shukla, Ram R. (Department: 1631)

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

C422S068100, C422S134000, C435S091200

Reexamination Certificate

active

10891161

ABSTRACT:
A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achieved by the present invention.

REFERENCES:
patent: 3616264 (1971-10-01), Ray et al.
patent: 4038055 (1977-07-01), Varano et al.
patent: 4072187 (1978-02-01), Lodge
patent: 4286456 (1981-09-01), Sista et al.
patent: 4420679 (1983-12-01), Howe
patent: 4599169 (1986-07-01), Ray
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis et al.
patent: 4701415 (1987-10-01), Dutton et al.
patent: 4865986 (1989-09-01), Coy et al.
patent: 4902624 (1990-02-01), Columbus et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4981801 (1991-01-01), Suzuki et al.
patent: 5038852 (1991-08-01), Johnson et al.
patent: 5139940 (1992-08-01), Isaacs et al.
patent: 5187084 (1993-02-01), Hallsby
patent: 3808942 (1989-09-01), None
patent: WO 8909437 (1989-10-01), None
Instruction manuel for the Perkin-Elmer Cetus DNA Thermal Cycler, Mar. 1988.
“Minimizing the Time Required for DNA Amplification by Efficient Heat Transfer to Small Samples,” Analytical Biochemistry, 186:328-331 (1990).
Cao, “A Simple and Inexpensive System to Amplify DNA by PCR,” Biotechniques, 7(6):566-567(1989).
Denton et al., “A Low-Cost Air-Driven Cycling Oven,” PCR Protocols: A Guide to Methods and Applications, 435-441 (1990).
Hoffman et al., “Use of a Gas Chromatograph Oven for DNA Amplification by the Polymerase Chain Reaction,” Biotechniques, 6(10):932-936 (1988).
Linz, “Thermocycler Temperature Variation Invalidates PCR Results,” Biotechniques, 9(3):286-290 (1990).
Wittwer et al., “Automated Polymerase Chain Reaction in Capillary Tubes with Hot Air,” Nucleic Acids Research, 17(11):4353-4357 (1989).
Instruction Manual for the Perkin-Elmer Celus DNA Thermal Cycler, Mar. 1988.
Morrison, et al. “Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization”Anal Biochem.183, pp. 231-244 (1989).
Coy Laboratory Products, Inc. Catalog page for “TempCycler”.
Eppendorf Advertisement for “Eppendorf MicroCycler™”.
Hybaid Advertisement for “Heating and Cooling Block”Products&Materials, Sep. 9, 1988, p. 1371.
Rollo F. , et al. “A simple and low cost DNA amplifer”Nucleic Acids Researchvol. 16, No. 7, (1988), pp. 3105-3106.
Wittwer, et al. Minimizing the Time Required for DNA Amplification by Efficient Heat Transfer to Small Samples:Anal.Biochem.(1990) vol. 186, pp. 328-331.

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