Multifunctional molecular complexes for gene transfer to cells

Details Multifunctional molecular complexes for gene transfer to cells Multifunctional molecular complexes for gene transfer to cells

2007-04-10

2007-04-10

Crouch, Deborah (Department: 1632)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C424S093200, C424S093210, C424S450000

Reexamination Certificate

active

10010114

ABSTRACT:
A multifunctional molecular complex for the transfer of a nucleic acid composition to a target cell is provided The complex is comprised of A) said nucleic acid composition and B) a transfer moiety comprising 1) one or more cationic polyamines bound to said nucleic acid composition, 2) one or more endosome membrane disrupting components attached to at least one nitrogen of the polyamine and 3) one or more receptor specific binding components.

REFERENCES:
patent: 5108921 (1992-04-01), Low
patent: 5166320 (1992-11-01), Wu
patent: 5171678 (1992-12-01), Behr
patent: 5283185 (1994-02-01), Epand
patent: 5614503 (1997-03-01), Chaudhary
patent: 5650096 (1997-07-01), Harris
patent: 5719131 (1998-02-01), Harris
patent: 5747471 (1998-05-01), Siegel
patent: 5767099 (1998-06-01), Harris
patent: 5783565 (1998-07-01), Siegel
patent: 5840710 (1998-11-01), Lee
patent: 5910487 (1999-06-01), Yew
patent: 5912239 (1999-06-01), Siegel
patent: 5925628 (1999-07-01), Lee
patent: 5935936 (1999-08-01), Fasbender
patent: 5942634 (1999-08-01), Siegel
patent: 5948767 (1999-09-01), Scheule
patent: 5981273 (1999-11-01), Curiel
patent: WO86/00930 (1986-02-01), None
patent: WO90/11092 (1990-10-01), None
patent: WO90/12095 (1990-10-01), None
patent: WO90/12096 (1990-10-01), None
patent: WO91/16024 (1991-10-01), None
patent: WO92/06180 (1992-04-01), None
patent: WO93/19768 (1993-10-01), None
patent: WO93/24640 (1993-12-01), None
Marshall, E. Science News: Gene Therapy's Growing Pains. Science. Aug. 25, 1995, vol. 269, No. 5227, pp. 1050-1055.
Anderson, W. F. Gene Therapy for Genetic Diseases. Human Gene Therapy. 1994, vol. 5, pp. 281-282.
Russell, S. J. Replicating Vectors for Gene Therapy of Cancer: Risks, Limitations and Prospects. Europ. J. Cancer. 1995, vol. 30A, No. 8, pp. 1165-1171.
Gutierrez, A. A. et al. Gene Therapy for Cancer. The Lancet. Mar. 21, 1992, vol. 339, pp. 715-7721.
Treco, D. A. et al. Non-Viral Gene Therapy. Molecular Medicine Today. 1995, vol. 1, pp. 314-321.
P. Atanasiu et al, “Virologie—Production de tumneurs chez le Hamster par inoculation d'acide desoxyribonucleique extrait de cultures de tissus infectees par le virus du polyome”, Academie des Sciences (Paris), 254:4228-4230 (1962).
F. Barthel et al, “Laboratory Methods, Gene Transfer Optimization with Lipospermine-Coated DNA”, DNA and Cell Biology, 12(6):553-560 (1993).
H. Basu et al, “Effects of Variation in the Structure of Spermine on the Association with DNA and the Induction of DNA Conformational Changes”, Biochem. J., 269:329-334 (1990).
J-P. Behr et al., “Efficient Gene Transfer into Mammalian Primary Endocrine Cells with Lipopolyamine-Coated DNA”, Proc. Natl. Acad. Sci. USA, 86:6982-6986 (Sep. 1989).
J-P. Behr, Gene Transfer with Synthetic Cationic Amphiphiles: Prospects for Gene Therapy, Bioconjugate Chem., 5:382-389 (1994).
P. Berman, “Efficacy of Recombinant Glycoprotein D Subunit Vaccines on the Development of Primary Recurrent, and Latent Genital Infections with Herpes Simplex Virus Type 2 in Guinea Pigs”, J. Infect Dis., 157(5):897-902 (May 1988).
C. Bennett et al, “Cationic Lipids Enhance Cellular Uptake and Activity of Phosphorothioate Antisense Oligonucleotides”, Molecular Pharmacology, 41:1023-1033 (1992).
H. Blau et al, “Molecular Medicine, Gene Therapy—A Novel Form of Drug Delivery”, New Engl. J. Med., 333(18):1204-1207 (Nov. 2, 1995).
J. Bodmer et al, “Carrier Potential of Glycoproteins”, Methods in Enzymology, 112:298-306 (1985).
E. Bonfils et al, “Uptake by Macrophages of a Biotinylated Oligo-α-deoxythymidylate by Using Mannosylated Streptavidin”, Bioconj. Chem., 3:277-284 (1992).
E. Bonfils et al, “Drug Targeting: Synthesis and Endocytosis of Oligonucleotide-Neoglycoprotein Conjugates”, Nucleic Acids Research, 20(17):4621-4629 (1992).
V. Chaudhary et al, “A Rapid Method of Cloning Functional Variable-Region Antibody Genes inEscherichia colias Single-Chain Immunotoxins”, Proc. Natl. Acad. Sci. USA, 87:1066-1070 (Feb. 1990).
C-J. Chu et al, “Efficiency of Cytoplasmic Delivery by pH-Sensitive Liposomes to Cells in Culture”, Pharmaceutical Research, 7(8):824-834 (1990).
G. Citro et al, “Inhibition of Leukemia Cell Proliferation by Receptor-Mediated Uptake of c-myb Antisense Oligodeoxynucleotides”, Proc. Natl. Acad. Sci. USA, 89:7031-7035 (Aug. 1992).
Cohen, “Oligonucleotides: Antisense Inhibitors of Gene Expression”, CRC Press, Inc., Boca Raton, FL (1989).
C. Connelly et al, “Polyamines Eliminate an Extreme Size Bias Against Transformation of Large Yeast Artificial Chromosome DNA”, Genomics, 10:10-16 (1991).
M. Cotten et al, “High-Efficiency Receptor-Mediated Delivery of Small and Large 48 kilobase Gene Constructs Using the Endosome-Disruption Activity of Defective or Chemically Inactivated Adenovirus Particles”, Proc. Natl. Acad. Sci. USA, 89:6094-6098 (Jul. 1992).
D. Curiel et al, “Adenovirus Enhancement of Transferrin-Polylysine-Mediated Gene Delivery”, Proc. Natl. Acad. Sci. USA, 88:8850-8854 (Oct. 1991).
S. Edgington, “Ribozymes: Stop Making Sense”, Biotechnology, 10:256-262 (Mar. 1992).
M. Elofsson et al, “Solid-Phase Synthesis and Conformational Studies of Glycosylated Derivaties of Helper-T-Cell Immunogenic Peptides from hen-egg Lysozyme”, Carbohydrate Research, 246:89-103 (1993).
J. Felgner et al, “Enhanced Gene Delivery and Mechanism Studies with a Novel Series of Cationic Lipid Formulations”, J. Biol. Chem., 269(4):2550-2561 (Jan. 1994).
P. Felgner et al, “Cationic Liposome-Mediated Transfection”, Nature, 337:387-388 (Jan. 1989).
L. Gao et al, “Direct In Vivo Gene Transfer to Airway Epithelium Employing Adenovirus-Polylysine-DNA Complexes”, Human Gene Therapy, 4:17-24 (1993).
H. Gershon et al, “Mode of Formation and Structural Features of DNA-Cationic Liposome Complexes Used for Transfection”, Biochemistry, 32:7143-7151 (1993).
J. Haensler et al, “Polyamidoamine Cascade Polymers Mediate Efficient Transfection of Cells in Culture”, Bioconjugate Chem., 4:372-379 (1993).
J. Haensler et al, “Synthesis and Characterization of a Trigalactosylated Bisacridine Compound to Target DNA to Hepatocytes”, Bioconjugate Chem., 4:85-93 (1993).
M. Howell et al, “Limited T-Cell Receptor β-chain Heterogeneity Among Interleukin 2 Receptor-Positive Synovial T Cells Suggests a Role for Superantigen in Rheumatoid Arthritis”, Proc. Natl. Acad. Sci. USA, 88:10921-10925 (Dec. 1991).
B. Huckett et al, “Evidence for Targeted Gene Transfer by Receptor-Mediated Endocytosis, Stable Expression Following Insulin-Directed Entry of NEO into HepG2 Cells”, Biochemical Pharmacology, 40(2):253-263 (1990).
M. Israel et al, “Biological Activity of Polyoma Viral DNA in Mice and Hamsters”, J. Virol., 39:990-996 (Mar. 1979).
J. Jones et al, “Cell Fusion Induced by the Murine Leukemia Virus Envelope Glycoprotein”, J. Virol., 67(1):67-74 (Jan. 1993).
R. Juliano et al, “Interaction of Polynucleotides with Cultured Mammalian Cells”, Experimental Cell Research, 73:3-12 (1972).
A. Kabanov et al, “Efficient Transformation of Mammalian Cells Using DNA Interpolyelectrolyte Complexes with Carbon Chain Polycations”, Bioconjugate Chem., 4:448-454 (1993).
Kabat et al, “1987 Sequence of Proteins of Immunological Interest U.S. Department of Health and Human Service”, Bethesda, MD.
K. Kawaguchi et al, “Differential Uptake of D-Glucosyl-neoglycoproteins by Isolated Rat Hepatocytes”, J. Biol. Chem., 256(5):2230-2234 (Mar. 1981).
K. Kono et al, “Fusion Activity of an Amphiphilic Polypeptide Having Acidic Amino Acid Residues: Generation of Fusion Activity by α-helix Formation and Charge Neutralization”, Biochimica et Biophysica Acta., 1164:81-90 (1993).
C. Leamon et al

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