Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-05-01
2007-05-01
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S070100, C435S071100
Reexamination Certificate
active
11145781
ABSTRACT:
The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.
REFERENCES:
patent: 4241050 (1980-12-01), Barth
patent: 4675285 (1987-06-01), Clark et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4728591 (1988-03-01), Clark et al.
patent: 4802951 (1989-02-01), Clark et al.
patent: 5069769 (1991-12-01), Fujimiya et al.
patent: 5137609 (1992-08-01), Manian et al.
patent: 5190632 (1993-03-01), Fujimiya et al.
patent: 5434272 (1995-07-01), Corrie et al.
patent: 5614386 (1997-03-01), Metzker et al.
patent: 6919179 (2005-07-01), Rothschild et al.
patent: 0234799 (1987-09-01), None
patent: WO 90/05785 (1990-05-01), None
Albarella, et al., “Monoadduct forming photochemical reagents for labeling nucleic acids for hybridization,”Nucleic Acids Research117(11):4293-4308 (1989).
Allen et al.,Gel Electrophoresis and Isoelectric Focusing of Proteins, Walter de Gruyter, New York 1984, pp. 17-62.
Amit, et al., Photosensitive Protecting Groups of Amino Sugars and Their Use in Glycoside Synthesis. 2-Nitrobenzyloxcarbonylamino and 6-Nitroveratryloxycarbonylamino Derivatives,J. Org. Chem. 39(2):192-196 (1974).
Antibodies: A Laboratory Manual(E. Harlow and D. Lane, editors, Cold Spring Harbor Laboratory Press), 1988, pp. 53, 72-73.
Bain et al., “Site-Specific Incorporation of Nonnatural Residues during In Vitro Protein Biosynthesis with Semisynthetic Aminoacyl-tRNAs,”Biochemistry30:5411-21 (1991).
Bain et al., “Site-Specific Incorporation of Non-Natural Residues into Peptides: Effect of Residue Structure on Suppression and Translation Efficiencies,”Tetrahedron47:2389-2400 (1991).
Baldini et al., “MischargingEscherichia colitRNAPhcwith L-4′- [Trifluoromethyl)-3H-diazirin-3-yl] phenylalaine, a Photoactivatable Analogue of Phenylalanine,”Biochemistry27:7951-7959 (1988).
Billington, et al., “Synthesis and Photochemistry of PhotolabileN-Glycine Derivatives and Efforts of One on the Glycine Receptor,”Biochemistry31:5500-5507 (1992).
Bruce and Uhlenbeck, “Specific Interaction of Anticodon Loop Residues with Yeast Phenylatanyl-tRNA Synthestase,”Biochemistry21:3921-3926 (1982).
Crowley et al., “The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation,”Cell73:1101-1115 (1993).
Current Protocols in Molecular Biology(F.M. Ausubel et al. editors, Wiley Interscience, 1993), pp. 10-16, 10-77.
Czworkowski et al., “Fluorescence Study of the Topology of Messenger RNA Bound to the 30S Ribosomal Subunit ofEscherichia coli,”Biochemistry30:4821-4830 (1991).
Dawson, et al., “Affinity Isolation of Transcriptionally Active Murine Erythroleukemia Cell DNA Using a Cleavable Biotinylated Nucleotide Analog,”J. Biological Chemistry264:12830-12837 (1989).
DiCesare et al., “A High-Sensitivity Electrochemiluminescence-Based Detection System for Automated PCR Product Quantitation,”BioTechniques15:152-59 (1993).
Felgner et al., “Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure,”Proc. Natl. Acad. Sci USA84:7413-17 (1987).
Hall, et al., “Mapping Labeled Sites inEscherichia coliRibosomal RNA: Distribution of Methyl Groups and Identification of a Photoaffinity-Labeled RNA Region Putatively at the Peptidyltransferase Center,”Biochemistry24:5702-5711 (1985).
Happ et al., “New Approach to the Synthesis of 2′(3′)-OAminoacyl Oligoribonucleotides,”J. Org. Chem.52:5387-91 (1987).
Hardesty et al., “Extension and Folding of Nascent Peptides on Ribosomes.”The Translational Apparatus, Nierhaus et al. ed: New York and London; Plenum Press. p. 347-358 (1993).
Hardesty et al., “Ribosome function determined by fluorescence,”Biochimie74:391-401 (1992).
Heckler et al., “T4 RNA Ligase Mediated Preparation of Novel “Chemically Misacylated” tRNAPhes,”Biochemistry23:1468-73 (1984).
Heckler et al., “Preparation of 2′(3′)-O-Acyl-pCpA Derivatives as Substrates for T4 RNA Ligase-Mediated “Chemical Aminoacylation”,”Tetrahedron40:87-94 (1984).
Herman, et al., “Affinity Chromatography of DNA Labeled with Chemically Cleavable Biotinylated Nucleotide Analogs,”Analytical Biochemistry156:48-55 (1986).
Hemmila, I.A.,Chemical Analysis“Applications of Fluorescence in Immunoassays”, (Wiley&Sons 1991) pp. 138-159.
Houlihan, et al., “Nitrobenzyl Ester Chemistry for Polymer Processes Involving Chemical Amplification,”Maromolecules21:2001-2006 (1988).
Hudson, “Methodological Implications of Simultaneous Solid-Phases Peptide Synthesis. 1. Comparison of Different Coupling Procedures,”J. Org. Chem.53:617-624 (1988).
Johnson et al., “Protein Synthesis and Secretion as seen by the Nascent Protein Chain,”The Translational Apparatus, Nierhaus et al. ed: New York and London; Plenum Press. p. 359-370 (1993).
Johnson et al., “N-Acetyllysine Transfer Ribonucleic Acid: A Biologically Active Analogue of Aminoacyl Transfer Ribonucleic Acids,”Biochemistry15:569-575 (1976).
Johnson, “Chemically Modified Aminoacyl-tRNA as a Probe of Ribosome Structure: The Synthesis and in vitro Activity of ε-N-actyl-Lys-tRNA,” 1973 Thesis Excerpts, University of Oregon, Eugene, Oregon.
Kalbag and Roeske, “A Photolabile Protecting Group for Histidine,”J. Am. Chem. Soc.97:440-441 (1975).
Karolin, J., et al., “Flourescene and Absorption Spectroscopic Properties of Dipyrrometheneboron Difluoride (BODIPY) Derivatives in Liquids, lipid Membranes and Proteins,”J. Am. Chem. Soc.116-:7801-7806 (1994).
Kozak, “Point Mutations Define a Sequence Flanking the AUG Initiator Codon that Modulates Translation by Eukaryotic Ribosomes,”Cell44:283-292 (1986).
Krafft, et al., “Photoactivable Fluorophores. 3. Synthesis and Photoactivation of Fluorogenic Difunctionalized Fluoresceins,”J. Am. Chem. Soc.110:301-303 (1988).
Krieg et al., “Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle,”Proc. Natl. Acad. Sci. USA83:8604-08 (1986).
Kudlicki et al., “Chaperone-dependent Folding and Activation of Ribosome-bound Nascent Rhodanese,”J. Mol. Biol.244:319-331 (1994).
Kurzchalia, T.V., et al., “tRNA-Mediated Labelling of Proteins with Biotin. A Nonradioactive Method for the Detection of Cell-Free Translation Products,”Eur. J. Biochem.172:663-668 (1988).
Lesnikowski and Jaworska, “Studies on Stereospecific Formation of P-Chiral Internucleotide Linkage. Synthesis of (Rp,Rp)- and (Sp,Sp)- Thymidyly(3′, 5′) Thymidylyl (3′,5′) Thymidine Dl(o,O-Phosphorothioate) using 2-Nitrobenzyl Group as a New S-Protection,”Tetrahedron Letters30 (29):3821-3824 (1989).
Ma et al., “In Vitro Protein Engineering Using Synthetic tRNAAlawith Different Anticodons,”Biochemistry32:7939-7945 (1993).
Markings and Tsien, “Caged Ni
Olejnik Jerzy
Rothschild Kenneth J.
Sonar Sanjay M.
Ketter James
Medlen & Carroll LLP
The Trustees of Boston University
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