Messenger RNA profiling: body fluid identification using...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C536S025410

Reexamination Certificate

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11050538

ABSTRACT:
This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.

REFERENCES:
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Absolutely RNA Nanoprep kit: Instruction Manual, Stratagene;□□ http://www.stratagene.com/products/displayProduct.aspx?pid=137.
Bauer et al, “Protamine mRNA as molecular marker for spermatozoa in semen stains,” International Journal of Legal Medicine, 2003, 117, 175-179.
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Alberts, Bruce, et al.Molecular Biology of the Cell, 4thed., (2002). Chapter II. Section 7. “The Molecular Genetic Mechanisms That Create Specialized Cell Types.” http://www.ncbi.nlm.nih.gov./entrez/query.fegi?cmd=Search&db=books&doptcmdl=GenBo . . . .
Bauer, M., et al.International Journal of Legal Medicine. vol. 117 pp. 175-179. (2003). “Protamine mRNA as Molecular Marker for Spermatozoa in Semen Stains.”
Gubin, Alexander, et al.Blood. vol. 97. No. 3. (2001). “Human Erythroid Porphobilinogen Deaminase Exists in 2 Splice Variants.”
Juusola, Jane, et al.Forensic Science International. vol. 135. p. 85-96. (2003). “Messenger RNA profiling: A Prototype Method to Supplant Conventional Methods for Body Fluid Identification.”
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