Methods and oligonucleotides for the detection of...

Details Methods and oligonucleotides for the detection of... Methods and oligonucleotides for the detection of...

Filed on

2007-07-10

Date issued

2007-07-10

Examiner

Myers, Carla J. (Department: 1634)

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Measuring or testing process involving enzymes or...

SubSubClass

Involving nucleic acid

Other Related Categories

C435S091200, C536S024320, C536S024300

Type

Reexamination Certificate

Status

active

Patent number

10178331

Description

ABSTRACT:
A method for detecting aSalmonellaspecies,E. coliO157:H7, orListeria monocytogenesis disclosed. The method involves amplifying a genomic nucleotide sequence of a corresponding species and detecting the amplification product. Various primers and probes that can be used in the method are also disclosed. In one embodiment, the amplification step of the method is accomplished by real-time PCR and the amplification product is detected by fluorescence resonance energy transfer using a pair of labeled polynucleotides.

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