Methods for assaying homocysteine

Details Methods for assaying homocysteine Methods for assaying homocysteine

2007-03-20

2007-03-20

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S007100, C435S007600, C435S007720, C435S007800, C435S007900

Reexamination Certificate

active

10043787

ABSTRACT:
The present invention relates to compositions and methods for assaying homocysteine (Hcy) and thus related moieties, e.g., S-adenosylhomocysteine (SAH) or adenosine. More particularly, assay methods that employ, mutant SAH hydrolase having binding affinity for Hcy, SAH or adenosine but has attenuated catalytic activity, are provided. The modified enzymes and fusion proteins containing the modified enzymes are also provided.

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