Sweet potato MADS-box promoter directing high level...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...

Reexamination Certificate

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C435S320100, C435S419000, C536S024100, C800S298000

Reexamination Certificate

active

11153023

ABSTRACT:
The present invention relates to a promoter directing high levels of expression of a gene in plant storage roots, derived from the sweet potato MADS-box gene, a vector directing high levels of expression of a gene in plant storage roots comprising the same and a transient assay method expressing a foreign gene transiently in plant storage roots using the same vector. The promoter according to the present invention can induce high levels of expression particularly in plant storage roots. Therefore the present invention is very useful for the development of transgenic plants to produce valuable materials in large quantities in plant storage roots.

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Ellis et al., (1987) Maize adh-1 promoter sequences contrl anerobic regulation: addition of upstream prmoter sequences from Constitutive genes is necessary for expression in tobacco. EMBO J 6:11-16.
Isolation of MADS-box genes from sweet potato (Ipomoea batatas(L.) Lam.) expressed specifically in vegetative tissues, S.H. Kim et al., Plant Cell. Physiol., Mar. 2002, vol. 43(3), pp. 314-322.
High-level expression of tuberous root storage protein genes of sweet potato in stems of plantlets grown in vitro on sucrose medium, T. Hattori et al., Plant Mol. Biol., Apr. 1990, vol. 14(4), pp. 595-604.
Pyk 10, a seedling and root specific gene and promoter fromArabidopsis thaliana, I. Nitz et al., Plant Science., Jul. 2001, vol. 161(2), pp. 337-346.
International Search Report dated Feb. 25, 2005, 4 pages.

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