Enzymatic cascade bioreactor

Details Enzymatic cascade bioreactor Enzymatic cascade bioreactor

2007-09-04

2007-09-04

Lilling, Herbert J. (Department: 1657)

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

C435S094000, C435S105000, C435S289100, C435S291400, C435S304100

Reexamination Certificate

active

11080901

ABSTRACT:
Disclosed is an apparatus and method for continuously converting sucrose to β-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and α-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

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