Methods of RNA and protein synthesis

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S091500, C435S252300, C536S025300

Reexamination Certificate

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10764581

ABSTRACT:
The present invention relates to methods for RNA and/or protein synthesis using in vitro or in vivo expression systems. More specifically, the present invention provides a method for RNA and/or protein synthesis characterized in that the concentration of alpha subunit of RNA polymerase, but not of other subunits, is increased in the cellular or cell-free system, comparing to its natural concentration existing in the cellular or cell-free system.

REFERENCES:
patent: WO 98/24891 (1998-06-01), None
patent: WO 00/63413 (2000-10-01), None
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Lesley et al. J Biol Chem. vol. 266, No. 4, pp. 2632-2638, Feb. 1991.
Bowrin, Brissette and Inouye. Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in anEscherichia colicell-free system. J Bacteriol. vol. 174, No. 20, pp. 6685-6687, Oct. 1992.
Savchenko et al. TheBacillus stearothermophilusargCJBD operon harbours a strong promoter as evaluated inEscherichia colicells. Gene. vol. 212, No. 2, pp. 167-177, Jun. 1998.
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Inouye et al. Up-promoter mutations in the lpp gene ofEscherichia coli.Nucleic Acids Research, vol. 13, No. 9, pp. 3101-3110, 1985.
Carbonetti et al., “Effect of Mutations Causing Overexpression of RNA Polymerase α Subunit on Regulation of Virulence Factors inBordetella pertussis”, Journal of Bacteriology, vol. 176, No. 23, pp. 7267-7273, 1994, XP-001022285.
Russo et al., “Alpha: The Cinderella Subunit of RNA Polymerase”, Journal of Biological Chemistry, American Society of Biological Chemists, vol. 267, NO. 21, pp. 14515-14518, 1992, XP-000864425.
Gourse et al., “UPs and downs in bacterial transcription initiation: the role of the alpha subunit of RNA polymerase in promoter recognition”, Molecular Microbiology, vol. 37, No. 4, pp. 687-695, 2000, XP-002182862.
Estrem et al., “Identification of an UP element consensus sequence for bacterial promoters”, Proc. Natl. Acad. Sci. USA; vol. 95, pp. 9761-9766, 1998, XP-002182863.

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